Department of Zoology, School of Sciences, Gujarat University, Ahmedabad, India.
Pharm Biol. 2012 Mar;50(3):326-31. doi: 10.3109/13880209.2011.608075. Epub 2011 Dec 2.
There has been enormous interest in the development of alternative medicines for the control of diabetes. Use of carbohydrate-hydrolyzing enzyme inhibitors proved to be an important strategy for the management of postprandial hyperglycemia by delaying the process of carbohydrate hydrolysis and absorption.
Three common traditional herbs, namely, stem bark of Terminalia arjuna (Combretaceae), seeds of Eugenia cumini (Myrtaceae), and leaves of Aegle marmelos (Rutaceae), were tested for their α-amylase inhibitory activities to establish antidiabetic potential.
The plant extracts (aqueous, 50%, and 100% methanol) obtained were subjected to an in vitro amylase inhibitory assay using starch as a substrate and pancreatic amylase as the enzyme. Statistical differences and linear regression analysis were performed using GraphPad prism 5 software.
The 50% methanol extracts of T. arjuna, E. cumini, and A. marmelos at a concentrations 50-500 μg/mL showed maximum percentage inhibition on amylase activity with IC(50) values of 302 ± 0.55, 632 ± 0.21, and 503 ± 0.28 μg/mL, respectively. However, the 100% methanol extracts of all the three plants showed the least inhibitory activity.
The results show that T. arjuna > E. cumini > A. marmelos have excellent inhibitory activity and, therefore, might be effective in lowering postprandial hyperglycemia.
人们对开发替代医学控制糖尿病产生了浓厚的兴趣。碳水化合物水解酶抑制剂的使用被证明是通过延迟碳水化合物水解和吸收过程来控制餐后高血糖的重要策略。
测试三种常见的传统草药，即Terminalia arjuna（使君子科）的树皮、Eugenia cumini（桃金娘科）的种子和Aegle marmelos（芸香科）的叶子，以评估它们对α-淀粉酶的抑制活性，从而确定其抗糖尿病的潜力。
使用淀粉作为底物和胰淀粉酶作为酶，对植物提取物（水提物、50%甲醇提取物和 100%甲醇提取物）进行体外淀粉酶抑制活性测定。使用 GraphPad prism 5 软件进行统计差异和线性回归分析。
浓度为 50-500μg/mL 的 T. arjuna、E. cumini 和 A. marmelos 的 50%甲醇提取物对淀粉酶活性的最大抑制百分比，IC50 值分别为 302±0.55、632±0.21 和 503±0.28μg/mL。然而，所有三种植物的 100%甲醇提取物显示出最小的抑制活性。
结果表明，T. arjuna>E. cumini>A. marmelos 具有优异的抑制活性，因此可能有效降低餐后高血糖。
