Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Denmark.
Scand J Clin Lab Invest. 2012 Apr;72(2):175-9. doi: 10.3109/00365513.2011.637130. Epub 2011 Dec 5.
Peptide hormones may occur in particularly low amounts in samples from small animals. Hence, in a rat microdialysis study conventional immunoassays were not sufficiently sensitive to measure gastrin in the dialysis samples. We therefore exploited the observation that antibodies raised against the homologous hormone cholecystokinin (CCK) occasionally bind gastrin peptides with significantly higher affinity than the proper ligand. The immunoassay thus established could detect 1.0 pmol/l in 15 μl microdialysate, which corresponds to 23 attomol gastrin. Such detection limit is five-fold lower than that obtained with the most avid conventional gastrin antibodies. The results may encourage similar approaches for other peptides using homologue-raised antibodies when supersensitivity is required.
肽类激素在小动物样本中的含量可能特别低。因此，在大鼠微透析研究中，常规免疫测定法的灵敏度不足以检测透析样本中的胃泌素。因此，我们利用了这样一种观察结果，即针对同源激素胆囊收缩素 (CCK) 产生的抗体偶尔会以比配体高得多的亲和力结合胃泌素肽。由此建立的免疫测定法可以在 15 μl 微透析液中检测到 1.0 pmol/l，相当于 23 个皮摩尔胃泌素。这种检测限比最亲和的常规胃泌素抗体低五倍。当需要超敏度时，这些结果可能会鼓励使用同源物升高的抗体对其他肽类进行类似的方法。
