Estevam Marcelo, Appoloni Carlos Roberto, Malvezi Aparecida Donizette, Tatakihara Vera Lúcia Hideko, Panis Carolina, Cecchini Rubens, Rizzo Luiz Vicente, Pinge-Filho Phileno
Laboratório de Física Nuclear Aplicada, Departamento de Física, Universidade Estadual de Londrina, Paraná, Brasil.
FEMS Immunol Med Microbiol. 2012 Apr;64(3):334-42. doi: 10.1111/j.1574-695X.2011.00917.x. Epub 2011 Dec 28.
Trypanosoma cruzi, the causative agent of Chagas' disease (CD), is a substantial public health concern in Latin America. Laboratory mice inoculated with T. cruzi have served as important animal models of acute CD. Host hypoferremic responses occur during T. cruzi infection; therefore, it has been hypothesized that T. cruzi requires iron for optimal growth in host cells and, unlike extracellular pathogens, may benefit from host hypoferremic responses. Recent technological improvements of X-ray fluorescence are useful for diagnostics or monitoring in biomedical applications. The goal of our study was to determine whether the iron availabilities in Swiss and C57BL/6 mice differ during the acute phase of T. cruzi infection and whether the availability correlates with oxidative stress in the susceptible and resistant phenotypes identified in these mice. Our results showed that the decrease in iron levels in the skin of resistant infected mice correlated with the increase in oxidative stress associated with anemia and the reduction in parasite burden.
克氏锥虫是恰加斯病（CD）的病原体，在拉丁美洲是一个重大的公共卫生问题。接种克氏锥虫的实验室小鼠已成为急性恰加斯病的重要动物模型。在克氏锥虫感染期间宿主会出现低铁血症反应；因此，有人推测克氏锥虫在宿主细胞中最佳生长需要铁，并且与细胞外病原体不同，它可能受益于宿主的低铁血症反应。X射线荧光技术最近的改进在生物医学应用的诊断或监测中很有用。我们研究的目的是确定在克氏锥虫感染急性期，瑞士小鼠和C57BL/6小鼠体内的铁利用率是否存在差异，以及这种利用率是否与在这些小鼠中鉴定出的易感和抗性表型中的氧化应激相关。我们的结果表明，抗性感染小鼠皮肤中铁水平的降低与贫血相关的氧化应激增加以及寄生虫负荷的减少有关。
