Department of Food and Nutrition, Obesity/Diabetes Research Institutes, Hoseo University, Asan, South Korea.
Chin J Integr Med. 2011 Dec;17(12):925-32. doi: 10.1007/s11655-011-0936-3. Epub 2011 Dec 3.
To investigate the effects of Chang-Chul-Eui-Ee-In-Tang ([see text], CCEET), modififi ed CCEET (MCCEET), and Semen Coicis (SC, a major component of CCEET) on energy and glucose homeostasis. The possible mechanism of action of CCEET was also determined.
A total of 100 Sprague Dawley female rats were randomly assigned to 5 groups, with 20 in each group. Rats in 4 groups were fed with a high fat diet supplementation (2 g/kg body weight), and water extracts of CCEET, MCCEET, SC, and cellulose (negative control), respectively. The last group was fed with a low-fat diet as a positive control.
CCEET and MCCEET decreased body weight and body fat (mesenteric and retroperitoneal fat) more than SC. This decrease was due to decreased energy intake and increased energy expenditure and fat oxidation. The improvement in energy homeostasis was associated with the enhancement of the hypothalamic leptin signalling pathway involving potentiating the phosphorylation of the signal transducer and activator of transcription-3, as well as attenuating the phosphorylation of 5' adenosine monophosphate-activated protein kinase (AMPK). Both CCEET and MCCEET improved glucose tolerance without changing serum insulin levels during an oral glucose tolerance test but MCCEET had a better effect than CCEET.
Both CCEET and MCCEET safely exerted anti-obesity effects by enhancing energy balance in female rats with diet-induced obesity; MCCEET showed a better effect on glucose homeostasis.
研究肠泰合剂（Chang-Chul-Eui-Ee-In-Tang，CCEET）、改良肠泰合剂（Modified Chang-Chul-Eui-Ee-In-Tang，MCCEET）和薏苡仁（CCEET 的主要成分之一，Semen Coicis，SC）对能量和葡萄糖代谢稳态的影响。还确定了 CCEET 的可能作用机制。
将 100 只 Sprague Dawley 雌性大鼠随机分为 5 组，每组 20 只。4 组大鼠分别给予高脂肪饮食补充（2 g/kg 体重），并分别给予 CCEET、MCCEET、SC 和纤维素（阴性对照）的水提取物。最后一组作为阳性对照给予低脂饮食。
CCEET 和 MCCEET 比 SC 更能降低体重和体脂肪（肠系膜和腹膜后脂肪）。这种减少是由于能量摄入减少和能量消耗增加以及脂肪氧化增加所致。能量代谢稳态的改善与增强下丘脑瘦素信号通路有关，涉及增强信号转导和转录激活因子 3 的磷酸化，以及减弱 5' 腺苷单磷酸激活蛋白激酶（AMPK）的磷酸化。CCEET 和 MCCEET 均改善了口服葡萄糖耐量试验期间的葡萄糖耐量，而不改变血清胰岛素水平，但 MCCEET 的效果优于 CCEET。
CCEET 和 MCCEET 通过增强肥胖饮食诱导的雌性大鼠的能量平衡，安全地发挥抗肥胖作用；MCCEET 在葡萄糖代谢稳态方面表现出更好的效果。
