Institute of Analytical Science/Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, Xi'an, Shaanxi 710069, People's Republic of China.
Appl Biochem Biotechnol. 2012 Feb;166(3):764-73. doi: 10.1007/s12010-011-9465-y. Epub 2011 Dec 3.
Thermally two-dimensional lattice graphene (GR) and biocompatibility chitosan (CS) act as a suitable support for the deposition of palladium nanoparticles (PdNPs). A novel hydrogen peroxide (H(2)O(2)) biosensor based on immobilization of hemoglobin (Hb) in thin film of CS containing GR and PdNPs was developed. The surface morphologies of a set of representative membranes were characterized by means of scanning electron microscopy and showed that the PdNPs are of a sphere shape and an average diameter of 50 nm. Under the optimal conditions, the immobilized Hb showed fast and excellent electrocatalytic activity to H(2)O(2) with a small Michaelis-Menten constant of 16 μmol L(-1), a linear range from 2.0 × 10(-6) to 1.1 × 10(-3) mol L(-1), and a detection limit of 6.6 × 10(-7) mol L(-1). The biosensor also exhibited other advantages, good reproducibility, and long-term stability, and PdNPs/GR-CS nanocomposites film would be a promising material in the preparation of third generation biosensor.
热二维格子石墨烯（GR）和生物相容性壳聚糖（CS）作为钯纳米粒子（PdNPs）沉积的合适载体。本文制备了一种基于血红蛋白（Hb）固定在含有 GR 和 PdNPs 的 CS 薄膜中的新型过氧化氢（H（2）O（2））生物传感器。通过扫描电子显微镜对一组代表性膜的表面形貌进行了表征，结果表明 PdNPs 呈球形，平均直径为 50nm。在最佳条件下，固定化 Hb 对 H（2）O（2）表现出快速且优异的电催化活性，米氏常数较小，为 16μmol L（-1），线性范围为 2.0×10（-6）至 1.1×10（-3）mol L（-1），检测限为 6.6×10（-7）mol L（-1）。该生物传感器还表现出其他优点，如良好的重现性和长期稳定性，PdNPs/GR-CS 纳米复合材料薄膜有望成为第三代生物传感器制备的有前途的材料。
