Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, P R China.
Proteomics. 2012 Jan;12(2):300-12. doi: 10.1002/pmic.201100214.
Microcystin-RR (MC-RR) is a commonly encountered cyanotoxin and receives increasing attention due to the risk of its bioaccumulation in aquatic animals like fish. This study investigated the protein profiles of zebrafish (Danio rerio) testes after intraperitoneal injection (i.p.) with 0.5 LD(50) (2000 μg/kg). MC-RR caused a noticeable damage to testicular ultrastructure, showing widened intercellular junction, distention of mitochondria. The testes showed a rapid response of its defense systems to the oxidative stress caused by MC-RR. This is the first to use a proteomic approach to obtain an overview of the effects of MC-RR on the testes of zebrafish. The proteomic results revealed that toxin exposure remarkably altered the abundance of 24 proteins that were involved in cytoskeleton assembly, oxidative stress, glycolysis metabolism, calcium ion binding and other biological functions. In conclusion, MC-RR damaged the testes and was toxic to the reproductive system of male zebrafish mainly through causing oxidative stress.
微囊藻毒素-RR（MC-RR）是一种常见的蓝藻毒素，由于其在鱼类等水生动物体内的生物蓄积风险，受到越来越多的关注。本研究通过腹腔注射（i.p.）0.5LD（50）（2000μg/kg）MC-RR 后，研究了斑马鱼（Danio rerio）睾丸的蛋白质图谱。MC-RR 对睾丸超微结构造成明显损伤，表现为细胞间连接加宽，线粒体肿胀。睾丸对 MC-RR 引起的氧化应激表现出快速的防御系统反应。这是首次使用蛋白质组学方法全面了解 MC-RR 对斑马鱼睾丸的影响。蛋白质组学结果表明，毒素暴露显著改变了 24 种参与细胞骨架组装、氧化应激、糖酵解代谢、钙离子结合和其他生物学功能的蛋白质的丰度。总之，MC-RR 通过引起氧化应激，破坏睾丸并对雄性斑马鱼的生殖系统产生毒性。
