莫洛尼鼠白血病病毒DNA体外整合的序列要求
Sequence requirements for integration of Moloney murine leukemia virus DNA in vitro.
作者信息
Bushman F D, Craigie R
机构信息
Laboratory of Molecular Biology, National Institute of Diabetes, and Digestive and Kidney Diseases, Bethesda, Maryland 20892.
出版信息
J Virol. 1990 Nov;64(11):5645-8. doi: 10.1128/JVI.64.11.5645-5648.1990.
Abstract
Normal replication of Moloney murine leukemia virus (MoMLV) requires the integration of a DNA copy of the viral RNA genome into a chromosome of the host. In this work, we characterize the DNA sequences at the ends of the linear proviral precursor that are required for integration in the presence of MoMLV integration protein in vitro. We found that nine bases of MoMLV DNA at each end of a linear model substrate were sufficient for near-maximal levels of integration and that four bases of MoMLV DNA at each end were sufficient for low levels of correct integration. We also found that a 3'-terminal A residue was preferred for integration. We infer from the limited DNA sequence requirements for integration that factors in addition to DNA sequence direct integration protein to act at the ends of the viral DNA.
摘要
莫洛尼鼠白血病病毒（MoMLV）的正常复制需要将病毒RNA基因组的DNA拷贝整合到宿主染色体中。在这项研究中，我们对线性前病毒前体末端的DNA序列进行了表征，这些序列是在体外存在MoMLV整合蛋白的情况下进行整合所必需的。我们发现，线性模型底物两端各九个碱基的MoMLV DNA足以实现接近最大水平的整合，而两端各四个碱基的MoMLV DNA足以实现低水平的正确整合。我们还发现，3'末端的A残基更有利于整合。我们从整合对DNA序列要求有限这一点推断，除了DNA序列外，还有其他因素引导整合蛋白作用于病毒DNA的末端。
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