Hashimoto H
Third Department of Internal Medicine, Ehime University School of Medicine.
Nihon Shokakibyo Gakkai Zasshi. 1990 Jun;87(6):1392-400.
To assess the metabolic characteristics of cirrhotic hepatocytes, a primary culture of hepatocytes was established using rat liver induced cirrhosis by CCl4 administration. Using this system, cell responsiveness to different metabolic and excretory stimuli was investigated and compared with a primary culture of normal healthy rat hepatocytes. Cirrhotic hepatocytes showed reduced protein synthesis in response to insulin and reduced urea synthesis in response to glucagon. However, DNA synthesis stimulated by insulin and EGF was significantly enhanced in cirrhotic hepatocytes. No significant difference was observed in the fluorescein diacetate excretion rate. Cirrhotic hepatocytes showed impairment of antipyrine metabolism and conjugation and excretion of unconjugated bilirubin. These results suggest indirectly that cirrhotic hepatocytes may be less functionally mature than normal healthy hepatocytes.
为评估肝硬化肝细胞的代谢特征，通过给予四氯化碳诱导大鼠肝脏产生肝硬化，进而建立了肝细胞原代培养体系。利用该体系，研究了细胞对不同代谢和排泄刺激的反应，并与正常健康大鼠肝细胞的原代培养进行比较。肝硬化肝细胞对胰岛素反应的蛋白质合成减少，对胰高血糖素反应的尿素合成减少。然而，胰岛素和表皮生长因子刺激的DNA合成在肝硬化肝细胞中显著增强。在荧光素二乙酸排泄率方面未观察到显著差异。肝硬化肝细胞显示出安替比林代谢以及未结合胆红素的结合和排泄受损。这些结果间接表明，肝硬化肝细胞的功能成熟度可能低于正常健康肝细胞。
