Levin R M, Wein A J
Invest Urol. 1979 Jul;17(1):75-7.
Using specific radioligand receptor binding assays, we have, for the first time, quantitated both alpha- and beta-adrenergic receptors in the musculature of the lower ureter, bladder body, posterior bladder base, and proximal urethra of the dog, and in the bladder base and body of the rabbit. For the beta receptor assay 3H-dihydroalprenolol was used as the ligand, and, for the alpha receptor assay, 3H-dihydroergocryptine was used. In the dog, there was a significantly greater density of beta receptors in the bladder body than in either the ureter or the posterior bladder base. The urethra had an intermediate density. The bladder base and the proximal urethra had a significantly greater density of alpha receptors than either the ureter or the bladder dome. The dissociation constant of beta receptors for DHA was approximately 9 nM for all tissues studied, and that of alpha receptors for DHE approximately 7 nM. A similar distribution of alpha-and beta adrenergic receptors was seen in the rabbit bladder.
我们首次运用特定的放射性配体受体结合试验，对犬的下输尿管、膀胱体、膀胱后基部和近端尿道以及兔的膀胱基部和膀胱体中的α-和β-肾上腺素能受体进行了定量分析。β受体分析采用³H-二氢阿普洛尔作为配体，α受体分析则采用³H-二氢麦角隐亭。在犬体内，膀胱体中β受体的密度显著高于输尿管或膀胱后基部。尿道的受体密度处于中间水平。膀胱基部和近端尿道中α受体的密度显著高于输尿管或膀胱顶部。在所研究的所有组织中，DHA与β受体的解离常数约为9 nM，DHE与α受体的解离常数约为7 nM。在兔膀胱中也观察到了α-和β-肾上腺素能受体的类似分布。
