• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

利用噬菌体展示技术鉴定对活化Rho特异的分子传感器。

Use of phage display for the identification of molecular sensors specific for activated Rho.

作者信息

Chinestra Patrick, Lajoie-Mazenc Isabelle, Faye Jean-Charles, Favre Gilles

机构信息

INSERM UMR 1037, Cancer Research Centre of Toulouse, Claudius Regaud Cancer Institute, University of Toulouse, Toulouse, France.

出版信息

Methods Mol Biol. 2012;827:283-303. doi: 10.1007/978-1-61779-442-1_19.

DOI:10.1007/978-1-61779-442-1_19
PMID:22144282
Abstract

We describe a phage display approach to select active Rho-specific scFv sensors. This in vitro technique allows preserving the antigen conformation stability all along the selection process. We used the GTP locked RhoBQ63L mutant as antigen against the Griffin.1 library composed of a human synthetic V(H) + V(L) scFv cloned in the pHEN2 phagemid vector. The method described here has permitted to identify an scFv that discriminates between the activated and the inactivated form of the Rho subfamily.

摘要

我们描述了一种用于筛选活性Rho特异性单链抗体片段（scFv）传感器的噬菌体展示方法。这种体外技术能够在整个筛选过程中保持抗原构象的稳定性。我们使用GTP锁定的RhoBQ63L突变体作为抗原，针对克隆于pHEN2噬菌粒载体中的人源合成V(H)+V(L) scFv组成的Griffin.1文库进行筛选。本文所述方法已成功鉴定出一种能够区分Rho亚家族激活形式和失活形式的scFv。

相似文献

1
Use of phage display for the identification of molecular sensors specific for activated Rho.利用噬菌体展示技术鉴定对活化Rho特异的分子传感器。
Methods Mol Biol. 2012;827:283-303. doi: 10.1007/978-1-61779-442-1_19.
2
One-step expression and purification of single-chain variable antibody fragment using an improved hexahistidine tag phagemid vector.使用改进的六聚组氨酸标签噬菌粒载体一步表达和纯化单链可变抗体片段。
Protein Expr Purif. 2009 Dec;68(2):190-5. doi: 10.1016/j.pep.2009.08.004. Epub 2009 Aug 13.
3
Identification of a GTP-bound Rho specific scFv molecular sensor by phage display selection.通过噬菌体展示筛选鉴定一种结合 GTP 的 Rho 特异性单链抗体片段分子传感器。
BMC Biotechnol. 2008 Mar 31;8:34. doi: 10.1186/1472-6750-8-34.
4
Selection of scFv Antibody Fragments Binding to Human Blood versus Lymphatic Endothelial Surface Antigens by Direct Cell Phage Display.通过直接细胞噬菌体展示筛选与人血及淋巴管内皮表面抗原结合的单链抗体片段
PLoS One. 2015 May 20;10(5):e0127169. doi: 10.1371/journal.pone.0127169. eCollection 2015.
5
Single-chain variable fragment (scFv) antibodies optimized for environmental analysis of uranium.单链可变片段 (scFv) 抗体经优化后可用于铀的环境分析。
Anal Chem. 2011 May 15;83(10):3717-24. doi: 10.1021/ac200159x. Epub 2011 Apr 20.
6
Isolation and characterization of phage-displayed single chain antibodies recognizing nonreducing terminal mannose residues. 2. Expression, purification, and characterization of recombinant single chain antibodies.识别非还原末端甘露糖残基的噬菌体展示单链抗体的分离与鉴定。2. 重组单链抗体的表达、纯化及鉴定
Biochemistry. 2007 Jan 9;46(1):263-70. doi: 10.1021/bi0618767.
7
Generation of high affinity human single-chain antibody against PreS1 of hepatitis B virus from immune phage-display antibody library.从免疫噬菌体展示抗体库中产生针对乙型肝炎病毒前S1的高亲和力人单链抗体。
Hepatobiliary Pancreat Dis Int. 2004 Feb;3(1):77-81.
8
[Isolation and identification of a human single chain Fv antibody against amyloid-beta 1-42 soluble oligomers from a human phage display library].[从人噬菌体展示文库中分离和鉴定抗β-淀粉样蛋白1-42可溶性寡聚体的人单链Fv抗体]
Sheng Wu Gong Cheng Xue Bao. 2009 Aug;25(8):1195-203.
9
Enhancement of scFv fragment reactivity with target antigens in binding assays following mixing with anti-tag monoclonal antibodies.在与抗标签单克隆抗体混合后，结合测定中scFv片段与靶抗原反应性的增强。
J Immunol Methods. 2004 Nov;294(1-2):23-35. doi: 10.1016/j.jim.2004.08.005.
10
Screening and evaluation of human single-chain fragment variable antibody against hepatitis B virus surface antigen.抗乙型肝炎病毒表面抗原人单链可变区片段抗体的筛选与鉴定
Hepatobiliary Pancreat Dis Int. 2006 May;5(2):237-41.

引用本文的文献

1
Protocol to select conformation-specific intracellular antibodies for targeted protein degradation in an engineered cell line.用于在工程细胞系中靶向蛋白质降解的针对构象特异性细胞内抗体的选择方案。
STAR Protoc. 2020 Dec 30;2(1):100249. doi: 10.1016/j.xpro.2020.100249. eCollection 2021 Mar 19.
2
NaLi-H1: A universal synthetic library of humanized nanobodies providing highly functional antibodies and intrabodies.NaLi-H1：一个提供高功能抗体和胞内抗体的人源化纳米抗体通用合成文库。
Elife. 2016 Jul 19;5:e16228. doi: 10.7554/eLife.16228.
3
Melanoma Expressed-CD70 Is Regulated by RhoA and MAPK Pathways without Affecting Vemurafenib Treatment Activity.
黑色素瘤表达的CD70受RhoA和MAPK信号通路调控，且不影响维莫非尼的治疗活性。
PLoS One. 2016 Feb 1;11(2):e0148095. doi: 10.1371/journal.pone.0148095. eCollection 2016.
4
Generation of a single chain antibody variable fragment (scFv) to sense selectively RhoB activation.用于选择性检测RhoB激活的单链抗体可变片段（scFv）的生成。
PLoS One. 2014 Nov 3;9(11):e111034. doi: 10.1371/journal.pone.0111034. eCollection 2014.
5
Antibody phage display libraries: contributions to oncology.抗体噬菌体展示文库：对肿瘤学的贡献。
Int J Mol Sci. 2012;13(5):5420-5440. doi: 10.3390/ijms13055420. Epub 2012 May 4.