Isolation and culture of astrocytes from the retina and optic nerve.
作者信息
Lukas Thomas J, Wang Ai Ling
机构信息
Molecular Pharmacology and Biological Chemistry Ward 8-200, Northwestern University, Chicago, IL, USA.
出版信息
Methods Mol Biol. 2012;814:105-15. doi: 10.1007/978-1-61779-452-0_8.
PMID:22144303
Abstract
Detailed methods for the preparation of optic nerve head (ONH) astrocytes from human donor eyes and retinal astrocytes from rat eyes are described. Included is the immunopanning method used for ONH astrocyte isolation as well as cell characterization. The isolation of purified retinal astrocytes is outlined as a method applicable to rodent and other mammalian retinas.
摘要
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