Skaper Stephen D
Department of Pharmacology and Anesthesiology, University of Padova, Padova, Italy.
Methods Mol Biol. 2012;846:131-45. doi: 10.1007/978-1-61779-536-7_12.
Glial cells play a key role in nervous system function, providing neurotrophic factor support to neurons as well as taking part in two-way neuron-glia signaling (e.g., neurotransmitter release). White matter-derived glia are important in certain neurodegenerative diseases involving axonal loss, for example in multiple sclerosis. Here we describe procedures for the preparation and culture of mixed nerve cells from postnatal rat optic nerve, followed by protocols which can serve for the purification of individual populations of glia from this tissue, namely O2A progenitors and oligodendrocytes, and astrocytes and astrocyte precursors.
神经胶质细胞在神经系统功能中发挥关键作用，为神经元提供神经营养因子支持，并参与神经元与神经胶质细胞的双向信号传导（例如神经递质释放）。源自白质的神经胶质细胞在某些涉及轴突丧失的神经退行性疾病中很重要，例如在多发性硬化症中。在这里，我们描述了从新生大鼠视神经制备和培养混合神经细胞的方法，随后介绍了可用于从该组织中纯化单个神经胶质细胞群体的方案，即少突胶质前体细胞和少突胶质细胞，以及星形胶质细胞和星形胶质细胞前体。
