鞘氨醇单胞菌属菌株SYK-6中参与β-芳基醚对映选择性裂解的第三个谷胱甘肽S-转移酶基因的表征
Characterization of the third glutathione S-transferase gene involved in enantioselective cleavage of the β-aryl ether by Sphingobium sp. strain SYK-6.
作者信息
Tanamura Kazuyuki, Abe Tomokuni, Kamimura Naofumi, Kasai Daisuke, Hishiyama Shojiro, Otsuka Yuichiro, Nakamura Masaya, Kajita Shinya, Katayama Yoshihiro, Fukuda Masao, Masai Eiji
机构信息
Department of Bioengineering, Nagaoka University of Technology, Niigata, Japan.
出版信息
Biosci Biotechnol Biochem. 2011;75(12):2404-7. doi: 10.1271/bbb.110525. Epub 2011 Dec 7.
The glutathione S-transferases, LigF and LigE, of Sphingobium sp. strain SYK-6 respectively play a role in cleavage of the β-aryl ether of (+)-(βS)-α-(2-methoxyphenoxy)-β-hydroxypropiovanillone (MPHPV) and (-)-(βR)-MPHPV. The ligP gene, which showed 59% similarity to ligE at the amino acid level, was isolated from SYK-6. LigP produced in Escherichia coli revealed enantioselectivity for (-)-(βR)-MPHPV, and ligE and ligP alone contributed to the degradation of (-)-(βR)-MPHPV in SYK-6.
鞘氨醇单胞菌属菌株SYK-6的谷胱甘肽S-转移酶LigF和LigE分别在(+)-(βS)-α-(2-甲氧基苯氧基)-β-羟基苯丙酮（MPHPV）和(-)-(βR)-MPHPV的β-芳基醚裂解中发挥作用。从SYK-6中分离出在氨基酸水平上与ligE具有59%相似性的ligP基因。在大肠杆菌中产生的LigP对(-)-(βR)-MPHPV表现出对映选择性，并且单独的ligE和ligP有助于SYK-6中(-)-(βR)-MPHPV的降解。
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