Adachi Osao, Hours Roque A, Shinagawa Emiko, Akakabe Yoshihiko, Yakushi Toshiharu, Matsushita Kazunobu
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Japan.
Biosci Biotechnol Biochem. 2011;75(12):2418-20. doi: 10.1271/bbb.110575. Epub 2011 Dec 7.
4-Keto-D-arabonate (D-threo-pent-4-ulosonate) and 4-keto-D-ribonate (D-erythro-pent-4-ulosonate) were prepared from D-arabinose and D-ribose by two successive reactions of membrane-bound enzymes, D-aldopentose 4-dehydrogenase and 4-keto-D-aldopentose 1-dehydrogenase of Gluconobacter suboxydans IFO 12528. Alternatively, they were prepared from D-arabonate and D-ribonate with another membrane-bound enzyme, D-pentonate 4-dehydrogenase. Analytical data confirmed the chemical structures of the 4-pentulosonates prepared. This is the first report of successful enzymatic synthesis of 4-pentulosonates.
4-酮基-D-阿拉伯糖酸（D-苏式戊-4-酮糖酸）和4-酮基-D-核糖酸（D-赤式戊-4-酮糖酸）由氧化葡萄糖杆菌IFO 12528的膜结合酶D-醛戊糖4-脱氢酶和4-酮基-D-醛戊糖1-脱氢酶，通过D-阿拉伯糖和D-核糖的两步连续反应制备而成。另外，它们也可由D-阿拉伯糖酸和D-核糖酸与另一种膜结合酶D-戊糖酸4-脱氢酶制备得到。分析数据证实了所制备的4-戊酮糖酸的化学结构。这是关于4-戊酮糖酸酶促合成成功的首次报道。
