Craig J M, Hawkins J M, Yamada T, Ganeshaguru K, Mehta A B, Secker-Walker L M
Department of Haematology, Royal Free Hospital and School of Medicine, London, U.K.
Leukemia. 1990 Oct;4(10):678-81.
Knowledge of the level of commitment of the target cell in hematological malignancies may have important therapeutic and prognostic implications. Cell lineage involvement was investigated in two cases presenting with acute lymphoblastic leukemia diagnosed on clinical and immunological findings and having the Philadelphia translocation t(9;22)(q34;q11). DNA from cells separated into mononuclear (lymphoid) and granulocytic fractions was hybridized with Philadelphia breakpoint-specific probes. This revealed that the breakpoint giving rise to the Philadelphia chromosome in case 1 was within the major breakpoint cluster region and in case 2 was in the first intron of the BCR gene. Rearrangement was found in the lymphoid but not the granulocyte fraction in each case. It is therefore concluded that the target cell for chromosomal change in these cases was a lymphoid committed progenitor cell, irrespective of breakpoint location.
了解血液系统恶性肿瘤中靶细胞的分化程度可能具有重要的治疗和预后意义。对两例根据临床和免疫学检查诊断为急性淋巴细胞白血病且伴有费城染色体易位t(9;22)(q34;q11)的病例进行了细胞系受累情况的研究。将分离为单核（淋巴样）和粒细胞组分的细胞DNA与费城染色体断裂点特异性探针进行杂交。结果显示，病例1中产生费城染色体的断裂点位于主要断裂点簇区域内，病例2的断裂点位于BCR基因的第一个内含子中。在每个病例的淋巴样组分而非粒细胞组分中发现了重排。因此得出结论，在这些病例中，染色体改变的靶细胞是已分化的淋巴样祖细胞，与断裂点位置无关。
