一种针对活性C端ADAMTS - 18片段的多克隆抗体。

A polyclonal antibody against active C-terminal ADAMTS-18 fragment.

作者信息

Dang Suying, Bu Dawei, Hong Tao, Zhang Wei

机构信息

Department of Medical Genetics, School of Medicine, Shanghai Jiao Tong University, PR China.

出版信息

Hybridoma (Larchmt). 2011 Dec;30(6):567-9. doi: 10.1089/hyb.2011.0055.

DOI:10.1089/hyb.2011.0055

PMID:22149285

Abstract

The human ADAMTS-18, a disintegrin and metalloproteinase with thrombospondin type-1 modules 18, is a secreted Zn-metalloproteinase. The C-terminal 385-amino acid fragment of ADAMTS-18 (AD18C) is highly effective at promoting platelet thrombus dissolution in vivo. Therefore, polyclonal antibody (pAb) against AD18C fragment should be able to keep platelet thrombus stability, which has direct clinical relevance. In this report, pAb against AD18C fragment was generated from rabbit immunized with AD18C recombinant protein (rAD18C). The pAb showed specific binding with rAD18C and natural ADAMTS-18 protein by ELISA and Western blot assay. It shortens the mouse tail bleeding time in a dose-dependent manner. Thus, anti-AD18C pAb contributes to the regulation of platelet thrombus stability.

摘要

人ADAMTS - 18是一种具有血小板反应蛋白1型结构域的解聚素和金属蛋白酶18，是一种分泌型锌金属蛋白酶。ADAMTS - 18的C末端385个氨基酸片段（AD18C）在体内促进血小板血栓溶解方面非常有效。因此，针对AD18C片段的多克隆抗体（pAb）应该能够维持血小板血栓的稳定性，这具有直接的临床相关性。在本报告中，用AD18C重组蛋白（rAD18C）免疫兔子产生了针对AD18C片段的pAb。通过ELISA和蛋白质印迹分析，该pAb与rAD18C和天然ADAMTS - 18蛋白表现出特异性结合。它以剂量依赖的方式缩短小鼠尾部出血时间。因此，抗AD18C pAb有助于调节血小板血栓的稳定性。