Kaminishi-Tanikawa Akiko, Kurita Masakazu, Okazaki Mutsumi, Kawaguchi Runa, Ihara Aki, Niikura Mamoru, Takushima Akihiko, Harii Kiyonori
Department of Plastic Surgery, Faculty of Medicine, Kyorin University, Tokyo, Japan.
J Plast Surg Hand Surg. 2011 Sep;45(4-5):219-25. doi: 10.3109/2000656X.2011.599539.
Dermal fibroblasts (DF) obtained from the superficial dermal layer and those from the deep dermal layer have different cellular functions. These differences are often associated with excessive scarring; they also influence early wound healing. We therefore investigated the differences between superficial and deep dermal fibroblasts with special emphasis on their contractile properties, and ability to produce connective tissue. We investigated their proliferation kinetics, ability to contract collagen lattices, and chronological mRNA expression of eight genes associated with wound healing. To estimate the changes in the differences between them during the early phase of wound healing, we investigated mRNA expression in bFGF supplemented medium because bFGF is a representative cytokine that is familiar to clinicians. Superficial DF proliferate faster than deep DF in culture, whereas deep DF are better at contracting collagen lattices than superficial ones. In realtime analysis of polymerase chain reaction, the expression of type I and III collagen, fibronectin, TGF β1 and β3, and connective tissue growth factor were higher in deep DF than in superficial DF, while the expression of TGF β2 was higher in superficial DF. After bFGF supplementation, the relative dominance of mRNA expression between superficial and deep DF remained constant except for the expression of collagenase. According to our analysis, deep DF are superior to superficial DF at promoting wound healing (particularly contraction and production of connective tissue). The intradermal distribution of DF is appropriate for efficient wound healing.
从真皮浅层获取的真皮成纤维细胞（DF）与从真皮深层获取的真皮成纤维细胞具有不同的细胞功能。这些差异通常与过度瘢痕形成有关；它们还会影响伤口早期愈合。因此，我们研究了浅层和深层真皮成纤维细胞之间的差异，特别强调它们的收缩特性以及产生结缔组织的能力。我们研究了它们的增殖动力学、收缩胶原晶格的能力以及与伤口愈合相关的八个基因的时序mRNA表达。为了评估在伤口愈合早期它们之间差异的变化，我们研究了在添加碱性成纤维细胞生长因子（bFGF）的培养基中的mRNA表达，因为bFGF是临床医生熟知的一种代表性细胞因子。在培养中，浅层DF比深层DF增殖更快，而深层DF在收缩胶原晶格方面比浅层DF更胜一筹。在聚合酶链反应的实时分析中，I型和III型胶原、纤连蛋白、转化生长因子β1和β3以及结缔组织生长因子在深层DF中的表达高于浅层DF，而转化生长因子β2在浅层DF中的表达更高。添加bFGF后，除了胶原酶的表达外，浅层和深层DF之间mRNA表达的相对优势保持不变。根据我们的分析，深层DF在促进伤口愈合（特别是收缩和结缔组织产生）方面优于浅层DF。真皮成纤维细胞的皮内分布有利于高效的伤口愈合。
