腹侧海马连合的低频刺激可减少慢性颞叶癫痫大鼠模型中的癫痫发作。
Low frequency stimulation of ventral hippocampal commissures reduces seizures in a rat model of chronic temporal lobe epilepsy.
机构信息
Department of Biomedical Engineering, Neural Engineering Center, Case Western Reserve University, Cleveland, Ohio 44106, USA.
出版信息
Epilepsia. 2012 Jan;53(1):147-56. doi: 10.1111/j.1528-1167.2011.03348.x. Epub 2011 Dec 9.
PURPOSE
To investigate the effects of low frequency stimulation (LFS) of a fiber tract for the suppression of spontaneous seizures in a rat model of human temporal lobe epilepsy.
METHODS
Stimulation electrodes were implanted into the ventral hippocampal commissure (VHC) in a rat post-status epilepticus (SE) model of human temporal lobe epilepsy (n = 7). Two recording electrodes were placed in the CA3 regions bilaterally and neural data were recorded for a minimum of 6 weeks. LFS (60 min train of 1 Hz biphasic square wave pulses, each 0.1 ms in duration and 200 μA in amplitude, followed by 15 min of rest) was applied to the VHC for 2 weeks, 24 h a day.
KEY FINDINGS
The baseline mean seizure frequency of the study animals was 3.7 seizures per day. The seizures were significantly reduced by the application of LFS in every animal (n = 7). By the end of the 2-week period of stimulation, there was a significant, 90% (<1 seizure/day) reduction of seizure frequencies (p < 0.05) and a 57% reduction during the period following LFS (p < 0.05) when compared to baseline. LFS also resulted in a significant reduction of hippocampal interictal spike frequency (71%, p < 0.05), during 2 weeks of LFS session. The hippocampal histologic analysis showed no significant difference between rats that received LFS and SE induction and those that had received only SE-induction. None of the animals showed any symptomatic hemorrhage, infection, or complication.
SIGNIFICANCE
Low frequency stimulation applied at a frequency of 1 Hz significantly reduced both the excitability of the neural tissue as well as the seizure frequency in a rat model of human temporal lobe epilepsy. The results support the hypothesis that LFS of fiber tracts can be an effective method for the suppression of spontaneous seizures in a temporal lobe model of epilepsy in rats and could lead to the development of a new therapeutic modality for human patients with temporal lobe epilepsy.
目的
研究低频刺激纤维束对颞叶癫痫（TLE）大鼠模型自发性癫痫发作的抑制作用。
方法
在颞叶癫痫（TLE）大鼠癫痫后（SE）模型中，将刺激电极植入腹侧海马连合（VHC）（n=7）。双侧 CA3 区放置两个记录电极，记录至少 6 周的神经数据。VHC 每天 24 小时进行 2 周的 LFS（60 分钟 1 Hz 双相方波脉冲，每个脉冲持续 0.1 毫秒，幅度为 200 μA）。
主要发现
研究动物的基线平均癫痫发作频率为每天 3.7 次。在每只动物（n=7）中，LFS 的应用均显著减少了癫痫发作。在刺激的 2 周期间结束时，与基线相比，癫痫发作频率显著降低了 90%（<1 次/天）（p<0.05），在 LFS 之后的期间内降低了 57%（p<0.05）。LFS 还导致海马间发性尖峰频率显著降低（71%，p<0.05），在 2 周的 LFS 期间。海马组织学分析显示，接受 LFS 和 SE 诱导的大鼠与仅接受 SE 诱导的大鼠之间无显著差异。没有动物出现任何症状性出血、感染或并发症。
意义
以 1 Hz 的频率施加的低频刺激显著降低了 TLE 大鼠模型中神经组织的兴奋性和癫痫发作频率。这些结果支持了纤维束 LFS 可以是抑制大鼠 TLE 模型中自发性癫痫发作的有效方法的假说，并可能导致开发一种新的治疗方法用于人类 TLE 患者。
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