Danilkina E B, Lomov Iu M, Kolesnikov N N, Lobanov V V, Vlasov V P, Cherkasova L R, Badalova I M
Mikrobiol Zh (1978). 1990 May-Jun;52(3):10-6.
The process of L-transformation and L-transformed state duration have been studied for their effect on variability of main characters of revertant cultures of choleric vibrions L-forms at the population level with the use of cloned cultures of the choleric vibrions. The study was conducted on two strains of the choleric vibrion of the eltor biovar in different periods of storage in the L-transformed state (1, 3, 6 months). It has been revealed that characters of the species and biovar remained stable despite the influence of L-transforming agents. The characters of clone cultures characterizing virulence (sensitivity to KhDF phages, hemolytic activity, toxin production and virulence for sucking rabbits proved to be subjected to variability to the greatest extent with simultaneous preservation of the toxin-production gene. A resistant change of the serovar (from Inaba to Ogava) is observed only in one revertant-subculture of the virulent strain.
利用霍乱弧菌的克隆培养物，在群体水平上研究了L型转化过程和L型转化状态持续时间对霍乱弧菌L型回复培养物主要特征变异性的影响。对埃尔托生物型霍乱弧菌的两个菌株在L型转化状态下不同储存期（1、3、6个月）进行了研究。结果表明，尽管受到L型转化因子的影响，物种和生物型的特征仍保持稳定。表征毒力的克隆培养物特征（对KhDF噬菌体的敏感性、溶血活性、毒素产生以及对吮兔的毒力）在毒素产生基因同时保留的情况下，受到的变异性影响最大。仅在有毒力菌株的一个回复亚培养物中观察到血清型的抗性变化（从稻叶型到小川型）。
