Department of Clinical Oncology and Center for Cancer Research, University of Hong Kong, HKSAR, Hong Kong, People's Republic of China.
Cancer Lett. 2012 Mar;316(1):39-45. doi: 10.1016/j.canlet.2011.10.020. Epub 2011 Oct 20.
The group 2 LIM domain protein, Cysteine-rich intestinal protein 2 (CRIP2) was found to play an important role in esophageal squamous cell carcinoma (ESCC) tumorigenesis. Subcellular fractionation studies show that CRIP2 is expressed in the nucleus. Real-time quantitative PCR shows CRIP2 expression is down-regulated in ESCC tissues and cell lines. Functional studies reveal that CRIP2 reduces colony formation, growth, and invasion abilities. Furthermore, over-expression of CRIP2 induces apoptosis through induction of active caspases 3 and 9 proteins. In conclusion, this study shows CRIP2 plays an important role in the development of ESCC.
组 2 LIM 结构域蛋白，富含半胱氨酸的肠蛋白 2（CRIP2）被发现于食管鳞状细胞癌（ESCC）的肿瘤发生中发挥重要作用。亚细胞分级分离研究表明 CRIP2 在细胞核中表达。实时定量 PCR 显示 CRIP2 在 ESCC 组织和细胞系中表达下调。功能研究表明，CRIP2 降低集落形成、生长和侵袭能力。此外，CRIP2 的过表达通过诱导活性半胱天冬酶 3 和 9 蛋白诱导细胞凋亡。总之，本研究表明 CRIP2 在 ESCC 的发展中发挥重要作用。
