Department of Biological Science, The University of Tulsa, 800 S. Tucker Dr, Tulsa, OK 74104, USA.
Arch Virol. 2012 Mar;157(3):405-12. doi: 10.1007/s00705-011-1184-8. Epub 2011 Dec 9.
The 3'-terminal region (1191 nt) containing part of the NIb gene, complete coat protein (CP) and poly-A tail of 64 papaya ringspot virus (PRSV-W) isolates collected during 2008-2009 from watermelon in commercial fields of four different counties of Oklahoma were cloned and sequenced. Nucleotide and amino acid sequence identities ranged from 95.2-100% and 97.1-100%, respectively, among the Oklahoman PRSV-W isolates. Phylogenetic analysis showed that PRSW-W isolates clustered according to the locations where they were collected within Oklahoma, and each cluster contained two subgroups. All subgroups of Oklahoman PRSV-W isolates were on separate branches when compared to 35 known isolates originating from other parts of the world, including the one reported previously from the USA. This study helps in our understanding about the genetic diversity of PRSV-W isolates infecting cucurbits in Oklahoma.
从俄克拉荷马州四个不同县的商业农田中的西瓜中收集到的，2008-2009 年间的 1191 个核苷酸（nt）的 3'末端区域（包含部分 NIb 基因、完整外壳蛋白（CP）和 64 个番木瓜环斑病毒（PRSV-W）分离株的 Poly-A 尾），被克隆并进行了测序。俄克拉荷马州 PRSV-W 分离株的核苷酸和氨基酸序列同一性分别为 95.2-100%和 97.1-100%。系统进化分析表明，PRSW-W 分离株根据在俄克拉荷马州的采集地点聚类，每个聚类包含两个亚群。与来自世界其他地区的 35 个已知分离株（包括之前从美国报道的分离株）相比，俄克拉荷马州 PRSV-W 分离株的所有亚群都在单独的分支上。这项研究有助于我们了解感染俄克拉荷马州葫芦科作物的 PRSV-W 分离株的遗传多样性。
