Christoffersen T, Dannevig L, Lysne S, Straume B, Melby K
Department of Ophthalmology, University Hospital, Tromsø.
NIPH Ann. 1990 Jun;13(1):3-9.
Specimens of Chlamydia trachomatis were treated either by five different detergents, by ultrasound sonication or by four different buffers before cultivation in cycloheximide-treated McCoy cells. The chlamydial yield by cultivation and the variance of the yield were compared to a standard method using 0.2 molar sucrose in phosphate buffered saline (2SP) without application of detergents or sonication. 2SP was superior to the other buffers. None of the detergents increased the chlamydial yield. Sonication at energy-levels above approximately 1200 Ws/ml reduced the chlamydial yield significantly. Sonication just below this threshold doubled the yield. None of the methods reduced the variance of the yield.
沙眼衣原体标本在接种于经放线菌酮处理的 McCoy 细胞培养之前，分别用五种不同的去污剂处理、超声处理或四种不同的缓冲液处理。将培养后的衣原体产量及其产量差异与使用不含去污剂或超声处理的 0.2 摩尔蔗糖磷酸盐缓冲盐水（2SP）的标准方法进行比较。2SP 优于其他缓冲液。没有一种去污剂能提高衣原体产量。能量水平高于约 1200 Ws/ml 的超声处理会显著降低衣原体产量。略低于此阈值的超声处理使产量增加了一倍。没有一种方法能降低产量的差异。
