Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, RJ, Brazil.
Antonie Van Leeuwenhoek. 2012 Mar;101(3):657-70. doi: 10.1007/s10482-011-9683-x. Epub 2011 Dec 8.
A 6,000 Da peptide, named CaTI, was isolated from Capsicum annuum L. seeds and showed potent inhibitory activity against trypsin and chymotrypsin. The aim of this study was to determine the effect of CaTI on Saccharomyces cerevisiae, Candida albicans, Candida tropicalis and Kluyveromyces marxiannus cells. We observed that CaTI inhibited the growth of S. cerevisiae, K. marxiannus as well as C. albicans and induced cellular agglomeration and the release of cytoplasmic content. No effect on growth was observed in C. tropicalis but morphological changes were noted. In the spot assay, different degrees of sensitivity were shown among the strains and concentrations tested. Scanning electron microscopy showed that S. cerevisiae, K. marxiannus and C. albicans, in the presence of CaTI, exhibited morphological alterations, such as the formation of pseudohyphae, cellular aggregates and elongated forms. We also show that CaTI induces the generation of nitric oxide and interferes in a dose-dependent manner with glucose-stimulated acidification of the medium mediated by H(+)-ATPase of S. cerevisiae cells.
从辣椒种子中分离得到一种 6000 Da 的肽，命名为 CaTI，它对胰蛋白酶和糜蛋白酶表现出很强的抑制活性。本研究旨在确定 CaTI 对酿酒酵母、白色念珠菌、热带假丝酵母和马克斯克鲁维酵母细胞的影响。我们观察到 CaTI 抑制了酿酒酵母、马克斯克鲁维酵母以及白色念珠菌的生长，并诱导细胞聚集和细胞质内容物的释放。在热带假丝酵母中未观察到生长的影响，但观察到形态变化。在点样试验中，在所测试的菌株和浓度中显示出不同程度的敏感性。扫描电子显微镜显示，在 CaTI 存在的情况下，酿酒酵母、马克斯克鲁维酵母和白色念珠菌表现出形态改变，如假菌丝形成、细胞聚集和伸长形式。我们还表明，CaTI 诱导一氧化氮的产生，并以剂量依赖的方式干扰由酿酒酵母细胞 H(+)-ATPase 介导的葡萄糖刺激的酸化。
