Niimi Tomoaki
Department of Bioengineering Sciences, Nagoya University, Nagoya, Japan.
Methods Mol Biol. 2012;824:307-15. doi: 10.1007/978-1-61779-433-9_15.
Leishmania tarentolae is a trypanosomatid protozoan parasite of the gecko, and has been established as a new eukaryotic expression system for the production of recombinant proteins. It seems that a protozoan parasite is a curious choice as the expression host; however, Trypanosomatidae are rich in glycoproteins with a pattern of glycosylation closely related to those in mammals and higher vertebrates. Thus, one of the main advantages of a L. tarentolae expression system is the mammalian-type posttranslational modification of target proteins. Although there are few examples of recombinant protein expression using this system, it can be an attractive alternative to using mammalian cells. This chapter presents an overview of the newly developed protein expression system based on L. tarentolae.
沙氏利什曼原虫是壁虎的一种锥虫目原生动物寄生虫，已被确立为用于生产重组蛋白的新型真核表达系统。作为表达宿主，选择一种原生动物寄生虫似乎有些奇怪；然而，锥虫科富含糖蛋白，其糖基化模式与哺乳动物和高等脊椎动物的密切相关。因此，沙氏利什曼原虫表达系统的主要优势之一是对目标蛋白进行哺乳动物类型的翻译后修饰。尽管使用该系统进行重组蛋白表达的例子很少，但它可能是使用哺乳动物细胞的一种有吸引力的替代方法。本章概述了基于沙氏利什曼原虫新开发的蛋白表达系统。
