National Institute for Cellular Biotechnology, Dublin City University, Dublin, Ireland.
Prostate. 2012 Aug 1;72(11):1193-9. doi: 10.1002/pros.22469. Epub 2011 Dec 7.
Radical prostatectomy cures the majority of men with clinically localized disease, but up to 30% of men relapse with rising serum PSA levels. Stage, Gleason grade, and pre-operative PSA levels are associated with outcome but do not accurately predict which individuals will relapse. MicroRNA (miRNA) levels are altered in cancer and are associated with progression of disease. The miR-200 family has roles in prostate cancer.
miR-200a levels were measured in 18 radical prostatectomy samples from men who did not relapse and from 18 who did relapse, matched for stage (all T3), grade, and PSA levels. A pair of cancer and normal prostate cell lines derived from the same radical prostatectomy specimen were transfected with miR-200a to determine the effects on growth, wound healing, and invasion.
Comparing the matched samples, 11 of the relapsers contained lower, 2 higher and 5 similar levels to the non-relapsers. Transient transfection of miR-200a significantly reduced cell proliferation in prostate cancer cell lines but did not affect invasiveness.
miR-200a overexpression reduced prostate cancer cell growth and may have potential, in combination with other markers, in stratifying prostate cancer patients for more intensive monitoring and therapy.
根治性前列腺切除术治愈了大多数患有临床局限性疾病的男性，但多达 30%的男性因血清 PSA 水平升高而复发。分期、Gleason 分级和术前 PSA 水平与预后相关，但不能准确预测哪些患者会复发。miRNA（miRNA）水平在癌症中发生改变，并与疾病的进展相关。miR-200 家族在前列腺癌中具有作用。
在 18 例无复发和 18 例复发的接受根治性前列腺切除术的男性的前列腺组织样本中测量了 miR-200a 的水平，这些患者的分期（均为 T3）、分级和 PSA 水平相匹配。从同一例根治性前列腺切除术标本中获得的一对癌细胞和正常前列腺细胞系用 miR-200a 转染，以确定对生长、伤口愈合和侵袭的影响。
比较匹配的样本，11 例复发者的 miR-200a 水平低于非复发者，2 例高于非复发者，5 例与非复发者相似。miR-200a 的瞬时转染显著降低了前列腺癌细胞系的增殖，但不影响侵袭性。
miR-200a 的过表达降低了前列腺癌细胞的生长，并且可能与其他标志物一起，在对前列腺癌患者进行更强化的监测和治疗方面具有潜力。
