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利用寡核苷酸修饰的抗污表面的多重电化学生物传感器检测大肠杆菌 uidA 基因上的单核苷酸多态性。

Detection of single-nucleotide polymorphism on uidA gene of Escherichia coli by a multiplexed electrochemical DNA biosensor with oligonucleotide-incorporated nonfouling surface.

机构信息

Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, China.

出版信息

Sensors (Basel). 2011;11(8):8018-27. doi: 10.3390/s110808018. Epub 2011 Aug 15.

DOI:10.3390/s110808018
PMID:22164059
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3231733/
Abstract

We report here a practical application of a multiplexed electrochemical DNA sensor for highly specific single-nucleotide polymorphism (SNP) detection. In this work, a 16-electrode array was applied with an oligonucleotide-incorporated nonfouling surfaces (ONS) on each electrode for the resistance of unspecific absorption. The fully matched target DNA templated the ligation between the capture probe assembled on gold electrodes and the tandem signal probe with a biotin moiety, which could be transduced to peroxidase-based catalyzed amperometric signals. A mutant site (T93G) in uidA gene of E. coli was analyzed in PCR amplicons. 10% percentage of single mismatched mutant gene was detected, which clearly proved the selectivity of the multiplexed electrochemical DNA biosensor when practically applied.

摘要

我们在此报告一种多重电化学 DNA 传感器的实际应用，该传感器可用于高度特异性的单核苷酸多态性 (SNP) 检测。在这项工作中，应用了一个 16 电极阵列，每个电极上都有一个带有寡核苷酸的非固着表面 (ONS)，以抵抗非特异性吸附。完全匹配的靶 DNA 模板化了在金电极上组装的捕获探针与带有生物素部分的串联信号探针之间的连接，这可以转化为基于过氧化物酶的催化安培信号。分析了大肠杆菌 uidA 基因中的一个突变位点 (T93G) 在 PCR 扩增子中。检测到 10%的单碱基错配突变基因，这清楚地证明了多重电化学 DNA 生物传感器在实际应用中的选择性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ec8/3231733/52f98b1327b5/sensors-11-08018f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ec8/3231733/b0ac914f42a7/sensors-11-08018f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ec8/3231733/3ad3ee6dc409/sensors-11-08018f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ec8/3231733/3acde5b7194e/sensors-11-08018f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ec8/3231733/5c81b9ec936a/sensors-11-08018f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ec8/3231733/924cdc9646d5/sensors-11-08018f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ec8/3231733/52f98b1327b5/sensors-11-08018f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ec8/3231733/b0ac914f42a7/sensors-11-08018f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ec8/3231733/3ad3ee6dc409/sensors-11-08018f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ec8/3231733/3acde5b7194e/sensors-11-08018f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ec8/3231733/5c81b9ec936a/sensors-11-08018f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ec8/3231733/924cdc9646d5/sensors-11-08018f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ec8/3231733/52f98b1327b5/sensors-11-08018f6.jpg

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