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[在辐射暴露条件下具有辐射适应性的杂色曲霉抗氧化系统的活性]

[Activity of antioxidant system of Aspergillus versicolor with radioadaptive properties under conditions of exposure to radiation].

作者信息

Tuhaĭ T I

出版信息

Mikrobiol Z. 2011 Sep-Oct;73(5):28-35.

PMID:22164697
Abstract

Melanin pigments were revealed for the first time in Aspergillus versicolor strains. Ionizing irradiation practically did not influence melanin synthesis in A. versicolor strains with radioadaptive properties and stimulated its synthesis in the control strain, which did not show such properties. High level of superoxide dismutase activity (from 100 up to 720 U/mg of protein) was revealed in the investigated strains. The irradiation in exponential phase of growth did not practically influence or raised activity of superoxide dismutase (SOD) in the strains with radioadaptive properties and oppressed its activity in the control strain. The irradiation in a stationary phase was accompanied by a decrease of SOD activity in the strains with radioadaptive properties, and essential (2 times) increase of SOD activity in the control strain and strain A. versicolor 101, which did not show radiotropism. The extracellular catalase activity (1.52-37.04 mmol x min(-1) x mg(-1) protein) exceeded by more than an order the intracellular one (8-230 mol x min(-1) x mg(-1) protein) in the investigated strains.

摘要

首次在杂色曲霉菌株中发现了黑色素色素。电离辐射对具有放射适应性的杂色曲霉菌株的黑色素合成几乎没有影响,却刺激了对照菌株(不具有此类特性)的黑色素合成。在所研究的菌株中发现了高水平的超氧化物歧化酶活性(从100到720 U/mg蛋白质)。在生长指数期进行辐射,对具有放射适应性的菌株的超氧化物歧化酶(SOD)活性几乎没有影响或使其活性升高,而抑制了对照菌株的该酶活性。在稳定期进行辐射,具有放射适应性的菌株的SOD活性降低,而对照菌株和不表现向放射性的杂色曲霉101菌株的SOD活性则显著(2倍)增加。在所研究的菌株中,细胞外过氧化氢酶活性(1.52 - 37.04 mmol·min⁻¹·mg⁻¹蛋白质)比细胞内过氧化氢酶活性(8 - 230 μmol·min⁻¹·mg⁻¹蛋白质)高出一个数量级以上。

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