Feng Ting, Xu Jing, Hang De-Rong, Wu Zi-Song, Tang Li, Wang Qi-Zhi, Yu Xin-Ling, Zeng Xiao-Jun, Shen Mei-Fen, Wu Cheng-Guo, Yuan Yi, Guo Jia-Gang, Zhou Xiao-Nong
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, China.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2011 Aug;23(4):370-6.
To understand the conditions of schistosomiasis laboratories at county level, so as to supply the information for diagnostic capacity building.
A questionnaire survey was conducted at 40 random selected counties which were in charge of national surveillance for schistosomiasis. The information of staffs, areas of laboratories, equipment configuration and diagnostic methods from each laboratory were collected. The serum specimens and Kato-Katz thick smears from the national surveillance sites were randomly selected and retested by provincial staffs to evaluate the testing ability of the personnel.
The average age of laboratory staffs was 40.93 +/- 9.56 years old, 69.39% of staffs were older than 35 years, 86.22% of them had education background below bachelor degree. Except missed professional titles of 4 persons, the staffs with primary, middle and high professional titles accounted for 56.63%, 39.29% and 2.04%, respectively. The laboratory areas varied greatly while independent schistosomiasis stations had the lowest areas with 52.81 +/- 40.08 m2, and the equipment configurations of laboratories were in a low level. The consistency rates of primary test and reexamination for serum specimens and thick smear slides were 95.89% and 99.53%, respectively, with the Kappa value over 0.90. Nine immunodiagnostic kits were used in these laboratories, and Kato-Katz technique and miracidium hatching technique were the main parasitological methods.
The personnel structure of laboratories at primary prevention and treatment facilities for schistosomiasis is unreasonable, while the basic infrastructure of laboratories is backward and the use of diagnostic assays/methods is disordered. The diagnostic capacity building should be strengthened, and the construction and management of schistosomiasis laboratories should be standardized.
了解县级血吸虫病实验室状况，为诊断能力建设提供信息。
对随机抽取的40个承担国家血吸虫病监测任务的县进行问卷调查，收集各实验室人员、面积、设备配置及诊断方法等信息。随机抽取国家监测点的血清标本和加藤厚涂片，由省级人员重新检测，以评估人员检测能力。
实验室人员平均年龄为40.93±9.56岁，69.39%的人员年龄大于35岁，86.22%人员学历低于本科。除4人未填写职称外，初级、中级和高级职称人员分别占56.63%、39.29%和2.04%。实验室面积差异较大，独立血吸虫病防治站面积最小，为52.81±40.08平方米，实验室设备配置水平较低。血清标本和厚涂片初筛与复检的符合率分别为95.89%和99.53%，Kappa值均大于0.90。这些实验室使用9种免疫诊断试剂盒，寄生虫学主要方法为加藤法和毛蚴孵化法。
血吸虫病基层防治机构实验室人员结构不合理，实验室基础设施落后，诊断试剂/方法使用混乱。应加强诊断能力建设，规范血吸虫病实验室的建设与管理。
