Chen Nian-Gao, Lin Dan-Dan, Xie Shu-Ying, Wang Qi-Zhi, Tang Li, Liu Yue-Min, Zeng Xiao-Jun, Liu Hong-Yun, Huang Mei-Jiao, Chen Hong-Gen
Jiangxi Institute of Parasitic Diseases, Nanchang 330046, China.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2011 Aug;23(4):377-80.
To evaluate the clinical diagnostic efficiency of Indirect Hemagglutination Assay Kit (IHA kit) for antibody detection of schistosomiasis japonica.
Schistosomiasis examinations by parasitological method were conducted in the schistosomiasis endemic areas in Jiangxi, Anhui and Hubei provinces, respectively. A total of 371 serum samples of schistosomiasis patients and 761 serum samples of non-patients without history of schistosome infection and infested water contact were collected. All the sera were detected by using IHA Kit. The diagnostic efficiency indicators of the kit, including sensitivity, specificity, positive predicted value, negative predicted value etc. were calculated.
The sensitivity and specificity of the kit were 95.1% (95% confidence interval was between 92.9% and 97.3%) and 97.8% (95% confidence interval was between 96.8% and 98.8%), respectively; the positive and negative predicted values were 95.4% and 97.6%, respectively. No statistically significant difference was found in the prevalence of IHA positive detecting by the kit among the groups with different EPG levels (< or = 40, 41-100, > 100). Also, no significant difference was observed in sensitivity and specificity among the different age groups.
The IHA kit shows a relative high efficiency for diagnosis and can be used for mass screening of schistosomiasis in field sites.
评估间接血凝试验试剂盒（IHA试剂盒）用于日本血吸虫病抗体检测的临床诊断效率。
分别在江西、安徽和湖北血吸虫病流行区采用寄生虫学方法进行血吸虫病检查。共收集371份血吸虫病患者血清样本和761份无血吸虫感染史及疫水接触史的非患者血清样本。所有血清均采用IHA试剂盒进行检测。计算该试剂盒的诊断效率指标，包括敏感性、特异性、阳性预测值、阴性预测值等。
该试剂盒的敏感性和特异性分别为95.1%（95%置信区间为92.9%至97.3%）和97.8%（95%置信区间为96.8%至98.8%）；阳性预测值和阴性预测值分别为95.4%和97.6%。在不同EPG水平（<或=40、41 - 100、>100）的组间，该试剂盒检测IHA阳性的患病率无统计学显著差异。不同年龄组间的敏感性和特异性也无显著差异。
IHA试剂盒显示出较高的诊断效率，可用于现场大规模筛查血吸虫病。
