Xue Mei, Jiang Zhen-Zhou, Wu Tao, Yan Ming, Liu Ji-Ping, Mu Xian-Min, Su Yu-Wen, Zhang Lu-Yong
Jiangsu Center for Drug Screening Laboratory, China Pharmaceutical University No. 24, Tongjiaxiang, Nanjing, Jiangsu Province, PR China.
Arzneimittelforschung. 2011;61(10):571-6. doi: 10.1055/s-0031-1300555.
The protective effects of tripterygium glycoside-loaded solid lipid nanoparticles (TG-SLNs) on male reproductive toxicity were investigated in rats. Thirty-six male Sprague-Dawley rats were randomly divided into three groups of 12: control group, tripterygium glycoside (TG) group, and TG-SLN group. After the animals had been orally administered with the substances for 28 consecutive days, their sperm count and sperm motility, organ coefficients, serum testosterone levels, testicular ultrastructure, and reproductive ability were observed. The results showed that the sperm motility rate in the TG group was only 3%, whereas the rates in the TG-SLN and control groups were 33% and 71%, respectively. Compared with those in the control group, the motion counts of path velocity, track speed, progressive velocity, straightness, linearity, beat cross frequency, amplitude of lateral head displacement, and sperm concentrations in the TG-SLN group were not significantly different while those in the TG group significantly decreased (p < 0.01). TG-SLNs did not cause testicular atrophy and instead maintained normal serum testosterone levels. The effect of TG-SLNs on the testicular ultrastructure was very evident; the morphologies of Sertoli, spermatogonial, mitochondrial, and sperm cells were normal. In terms of reproductive ability, one rat (17%) from the TG-SLN group and five rats (83%) from the control group became pregnant, whereas none of the rats from the TG group became pregnant. These data indicate that TG-SLNs have potentially protective effects on male reproductive toxicity in rats.
研究了雷公藤多苷负载固体脂质纳米粒（TG-SLNs）对大鼠雄性生殖毒性的保护作用。将36只雄性Sprague-Dawley大鼠随机分为3组，每组12只：对照组、雷公藤多苷（TG）组和TG-SLN组。动物连续28天口服给予相应物质后，观察其精子计数、精子活力、器官系数、血清睾酮水平、睾丸超微结构和生殖能力。结果显示，TG组精子活力率仅为3%，而TG-SLN组和对照组分别为33%和71%。与对照组相比，TG-SLN组的路径速度、轨迹速度、前进速度、直线度、线性度、鞭打交叉频率、头部侧向位移幅度和精子浓度的运动计数无显著差异，而TG组显著降低（p<0.01）。TG-SLNs未导致睾丸萎缩，反而维持了正常的血清睾酮水平。TG-SLNs对睾丸超微结构的影响非常明显；支持细胞、精原细胞、线粒体和精子细胞的形态正常。在生殖能力方面，TG-SLN组有1只大鼠（17%）怀孕，对照组有5只大鼠（83%）怀孕，而TG组无大鼠怀孕。这些数据表明，TG-SLNs对大鼠雄性生殖毒性具有潜在的保护作用。
