磷酸钙水泥和重组人骨形态发生蛋白-2 复合材料重建兔眼眶缺损。

Reconstruction of orbital defect in rabbits with composite of calcium phosphate cement and recombinant human bone morphogenetic protein-2.

机构信息

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong 510060, China.

出版信息

Chin Med J (Engl). 2010 Dec;123(24):3658-62.

PMID:22166647

Abstract

BACKGROUND

Calcium phosphate cement (CPC) is a biocompatible and osteoconductive bone substitute, and recombinant human bone morphogenetic protein-2 (rhBMP-2) has strong osteoinductibility, therefore we developed a composite bone substitute with CPC and rhBMP-2 and evaluate its reconstruction effect in rabbit orbital defect.

METHODS

Thirty-six rabbits were randomly divided into two groups and a 5 mm × 5 mm × 2 mm bone defect in the infraorbital rim was induced by surgery in each orbit (72 orbits in all). The orbital defects were treated with pure CPC or composite of CPC and rhBMP-2. The osteogenesis ability of different bone substitute was evaluated by gross observation, histological examination, histomorphometrical evaluation, compressive load-to-failure testing, and scanning electron microscope (SEM).

RESULTS

Gross observation showed that both bone substitutes were safe and effective for reconstruction of orbital defect. However, histological examination, histomorphometrical evaluation and SEM showed that CPC/rhBMP-2 group had faster speed in new bone formation and degradation of substitute material than CPC group. Compressive load-to-failure testing showed that CPC/rhBMP-2 group had stronger compressive strength than CPC group at every stage with significant difference (P < 0.05).

CONCLUSION

Composite of CPC/rhBMP-2 is an ideal bioactive material for repairing orbital defect, with good osteoconductibility and osteoinductibility.

摘要

背景

磷酸钙骨水泥（CPC）是一种生物相容性和骨传导性的骨替代物，重组人骨形态发生蛋白-2（rhBMP-2）具有很强的成骨诱导性，因此我们开发了一种 CPC 和 rhBMP-2 的复合骨替代物，并评估其在兔眼眶缺损中的重建效果。

方法

36 只兔子被随机分为两组，在每个眼眶的眶下缘诱导 5mm×5mm×2mm 的骨缺损（总共 72 个眼眶）。用纯 CPC 或 CPC 和 rhBMP-2 的复合物治疗眼眶缺损。通过大体观察、组织学检查、组织形态计量学评估、压缩负载破坏测试和扫描电子显微镜（SEM）评估不同骨替代物的成骨能力。

结果

大体观察表明，两种骨替代物均安全有效，可用于眼眶缺损的重建。然而，组织学检查、组织形态计量学评估和 SEM 显示，CPC/rhBMP-2 组在新骨形成和替代材料降解方面的速度比 CPC 组更快。压缩负载破坏测试显示，CPC/rhBMP-2 组在每个阶段的抗压强度均高于 CPC 组，差异有统计学意义（P<0.05）。