Ichinohe Norihisa, Kon Junko, Mitaka Toshihiro
Department of Tissue Development and Regeneration, Research Institute for Frontier Medicine, Sapporo Medical University School of Medicine, Sapporo, Japan.
Methods Mol Biol. 2012;826:49-58. doi: 10.1007/978-1-61779-468-1_5.
Oval cells and small hepatocytes (SHs) are well known as hepatic stem/progenitor cells. However, the relationship between the oval cells and SHs in liver regeneration is not well understood. To resolve this issue, we established a technique to selectively separate oval cells and SHs. In the injured rat liver, oval cells and SHs transiently appear in the initial period of liver regeneration. Thy1(+) and CD44(+) cells are candidates for markers of oval cells and SHs, respectively. In this chapter, the methods for sorting and culture of the cells are described in detail.
卵圆细胞和小肝细胞（SHs）作为肝干细胞/祖细胞广为人知。然而，肝再生过程中卵圆细胞与小肝细胞之间的关系尚未完全明确。为解决这一问题，我们建立了一种选择性分离卵圆细胞和小肝细胞的技术。在受损的大鼠肝脏中，卵圆细胞和小肝细胞在肝再生初期短暂出现。Thy1(+)细胞和CD44(+)细胞分别是卵圆细胞和小肝细胞的标志物候选者。在本章中，将详细描述细胞的分选和培养方法。
