Ji Junfang, Wang Xin Wei
Laboratory of Human Carcinogenesis, Bethesda, MD, USA.
Methods Mol Biol. 2012;826:163-75. doi: 10.1007/978-1-61779-468-1_14.
Cancer Stem cells (CSCs) are the source of many solid tumor types including hepatocellular carcinoma. MicroRNAs (miRNAs) are small noncoding RNAs and have been showed to be associated with hepatic CSCs. Here, we described methods to screen hepatic CSC-related miRNAs, and to validate and examine their expressions and functions in vitro and in vivo, which contribute to the maintenance of stemness and differentiation of hepatic CSCs.
癌症干细胞（CSCs）是包括肝细胞癌在内的多种实体瘤类型的来源。微小RNA（miRNAs）是小的非编码RNA，已被证明与肝脏癌症干细胞有关。在这里，我们描述了筛选肝脏癌症干细胞相关微小RNA的方法，并在体外和体内验证和检测它们的表达及功能，这些方法有助于维持肝脏癌症干细胞的干性和分化。
