Liu Hsiang-Chao, Hwu Ching-Shyung, Hung Jui-Min, Lai Hung-Yu, Chang Hung-Yueh, Lu Chih-Jen
Department of Environmental Engineering, National Chung Shing University, No.250, Kuo Kuang Road, Taichung, Taiwan - 40227, ROC.
J Environ Biol. 2011 May;32(3):319-23.
Solid phase microextraction (SPME) coupled with gas chromatography was employed to estimate bioavailability of pyrene in soils with different properties of textures, organic matter contents (SOM) and aging periods. Experimental results indicated that biodegradation rates increased from 0.10 (sandy loam) to 0.15 (silty loam) microg g-1 hr1. By contrast, biodegradation rate decreased from 0.10(1.3% SOM) to 0.04 (7.6% SOM) microg g-1hr1. The amounts of pyrene biodegraded decreased 27% when SOM was modified from 1.3 to 7.6%, indicating that distributions of pyrene in soils at biodegradation end points were affected by the SOM. Sequestration as measured by sonication extraction had evidently occurred in aged soil samples. SPME measurements slightly overestimated the amount of pyrene degraded by indigenous and seeded microorganisms, in soils with the different properties (correlation coefficient, R2= 0.74). The present study demonstrates that the SPME method can not replace biodegradation tests commonly used for predicting bioremediation efficacy.
采用固相微萃取（SPME）与气相色谱联用的方法，评估芘在质地、有机质含量（SOM）和老化时间不同的土壤中的生物可利用性。实验结果表明，生物降解速率从0.10（砂壤土）增加到0.15（粉质壤土）μg g-1 hr-1。相比之下，生物降解速率从0.10（1.3% SOM）降至0.04（7.6% SOM）μg g-1hr-1。当SOM从1.3% 变为7.6%时，芘的生物降解量减少了27%，这表明芘在生物降解终点时在土壤中的分布受SOM影响。通过超声提取测定的螯合作用在老化土壤样品中明显发生。在不同性质的土壤中，SPME测量略微高估了土著和接种微生物降解的芘的量（相关系数，R2 = 0.74）。本研究表明，SPME方法不能替代通常用于预测生物修复效果的生物降解试验。
