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[对具有支链淀粉型淀粉的育种面包小麦品系的分子遗传学分析]

[Molecular-genetic analysis of the breeding bread wheat lines with starch of amylopectin type].

出版信息

Tsitol Genet. 2011 Sep-Oct;45(5):17-22.

Abstract

PCR-analysis with primers to Wx-loci of T. aestivum L. genome lets us run the controlled selection of genotypes with null-alleles of Wx-genes while creating bread wheat lines with amylopectin type of starch. Microsatellite analysis of selected individual plants which were the carries of three null-alleles of Wx-genes (Wx-A1b, Wx-B1b, Wx-D1b) and the cluster analysis in the complex allowed us to pick out four genotypes, which were very close related genetically to the parent form variety Kuyalnik.

摘要

利用针对普通小麦L基因组Wx位点的引物进行PCR分析,能让我们在培育具有支链淀粉型淀粉的面包小麦品系时,对携带Wx基因无效等位基因的基因型进行可控选择。对携带Wx基因三个无效等位基因(Wx-A1b、Wx-B1b、Wx-D1b)的选定单株进行微卫星分析,并在复合体中进行聚类分析,使我们能够挑选出四种基因型,它们在遗传上与亲本品种库亚尔尼克非常接近。

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