通过改变银胶体悬浮液的 pH 值来增加 RNA 和 DNA 成分的表面增强拉曼光谱效应。

Increasing surface enhanced Raman spectroscopy effect of RNA and DNA components by changing the pH of silver colloidal suspensions.

机构信息

Center for Sensor Development/Chemical Imaging Center/ALERT DHS-COE, Department of Chemistry, University of Puerto Rico-Mayaguez, P.O. Box 9019, Mayaguez, PR 00681, Puerto Rico.

出版信息

Spectrochim Acta A Mol Biomol Spectrosc. 2012 Feb 15;87:77-85. doi: 10.1016/j.saa.2011.11.012. Epub 2011 Nov 18.

DOI:10.1016/j.saa.2011.11.012

PMID:22169024

Abstract

This work focused on establishing the parameters for enhancing the Raman signals of DNA and RNA constituents: nitrogenous bases, nucleosides and nucleotides, using metallic nanoparticles as surface enhanced Raman scattering substrates. Silver nanospheres were synthesized using sodium borohydride as a reducing agent and sodium citrate as a capping agent. The prepared nanoparticles had a surface plasmon band at ∼384nm and an average size of 12±3nm. The nanoparticles' surface charge was manipulated by changing the pH of the Ag colloidal suspensions in the range of 1-13. Low concentrations as 0.7μM were detected under the experimental conditions. The optimum pH values were: 7 for adenine, 9 for AMP, 5 for adenosine, 7 for dAMP and 11 for deoxyadenosine.

摘要

这项工作专注于建立使用金属纳米粒子作为表面增强拉曼散射基底来增强 DNA 和 RNA 成分（含氮碱基、核苷和核苷酸）拉曼信号的参数。使用硼氢化钠作为还原剂和柠檬酸钠作为封端剂合成了银纳米球。所制备的纳米粒子在 ∼384nm 处具有表面等离子体带，平均尺寸为 12±3nm。通过改变 Ag 胶体悬浮液的 pH 值在 1-13 范围内来控制纳米粒子的表面电荷。在实验条件下检测到低浓度为 0.7μM。最佳 pH 值分别为：腺嘌呤为 7、AMP 为 9、腺苷为 5、dAMP 为 7 和脱氧腺苷为 11。