缺失内质网 1,2-α-甘露糖苷酶活性，导致米曲霉 manE 基因敲除突变株中 N-糖链 Man9GlcNAc2 修剪为 Man8GlcNAc2 异构体 B。
Lack of endoplasmic reticulum 1,2-α-mannosidase activity that trims N-glycan Man9GlcNAc2 to Man8GlcNAc2 isomer B in a manE gene disruptant of Aspergillus oryzae.
机构信息
National Research Institute of Brewing, 3-7-1 Kagamiyama, Higashi-hiroshima, Hiroshima 739-0046, Japan.
出版信息
J Biosci Bioeng. 2012 Apr;113(4):438-41. doi: 10.1016/j.jbiosc.2011.11.015. Epub 2011 Dec 13.
PMID:22169093
Abstract
The gene manE, encoding a probable class I endoplasmic reticulum 1,2-α-mannosidases (ER-Man), was identified from the filamentous fungus Aspergillus oryzae due to similarity to orthologs. It removes a single mannose residue from Man(9)GlcNAc(2), generating Man(8)GlcNAc(2) isomer B. Disruption of manE caused drastic decreases in ER-Man activity in A. oryzae microsomes.
摘要
该基因 manE 编码一种可能的Ⅰ类内质网 1,2-α-甘露糖苷酶（ER-Man），由于与同源物相似，从丝状真菌米曲霉中鉴定出来。它从 Man(9)GlcNAc(2)中去除一个甘露糖残基，生成 Man(8)GlcNAc(2)异构体 B。在米曲霉微粒体中，manE 的缺失导致 ER-Man 活性急剧下降。
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