Gu Shu-Cheng, Chang Chun-Kang
Department of Hematology, Shanghai Jiaotong University, Shanghai, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2011 Dec;19(6):1545-9.
The next-generation sequencing (NGS), as the most practical and reliable method, has replaced the classical Sanger sequencing to help scientists to discover the genetics secrets of human tumor diseases. With the technique development, the whole genome sequencing will be no longer out of reach. Recently, some scientists used the NGS in the research of hematological malignancies and pushed the progress of the whole genome sequencing in acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS) actively in order to find out the pathogenesis of some hematological malignancies. The NGS and the application of the whole genome sequencing, the exome sequencing, the transcriptome sequencing in AML and MDS are reviewed in this article.
作为最实用且可靠的方法,新一代测序(NGS)已取代经典的桑格测序,帮助科学家发现人类肿瘤疾病的遗传学奥秘。随着技术的发展,全基因组测序将不再遥不可及。最近,一些科学家将NGS用于血液系统恶性肿瘤的研究,并积极推动急性髓系白血病(AML)和骨髓增生异常综合征(MDS)全基因组测序的进展,以找出某些血液系统恶性肿瘤的发病机制。本文对NGS以及全基因组测序、外显子组测序、转录组测序在AML和MDS中的应用进行了综述。