Lin Ting-ting, Zhu Li-min, He Yan-jin, Zhang Hong
Tianjin Medical University Eye Center, Tianjin, China.
Zhonghua Yan Ke Za Zhi. 2011 Aug;47(8):703-8.
To observe the expression and distribution of CD44, CD133, and ABCG2 in orbital adenoid cystic carcinoma (ACC) and investigate their correlations with pathological type and prognosis.
Two steps method of immunohistochemical staining was employed in 33 cases of paraffin embedded surgical specimens of human orbital ACC, 5 cases of recurrence samples, 3 cases of an excised lacrimal gland caused by neither inflammation nor tumor diseases, and 6 cases of xenograft tumors in nude mice. A retrospective analysis was performed on the clinical material of these patients, which were collected from Jan. 1991 to Mar. 2009.
The positive rate of CD44 was 54.5% (18/33), with 76.9% (10/13) in solid type and 40.0% (8/20) in adeno-tubiform type. There was no statistically significant difference between them (P = 0.072). In solid type the positive expression cells were often located at the marginal part of the cancer nest. In the adeno-tubiform type, positive cells were often located at the outer layer of the tubiform structure (myoepithelial cells). CD44 was also expressed in normal tissues. The positive rate of CD133 was 57.6% (19/33), with 76.9% (10/13) in solid type and 45.0% (9/20) in adeno-tubiform type. There was no significant difference between them (P = 0.087). CD133 antigen was expressed in either the cytoplasm or nucleus, or expressed in both the cytoplasm and nucleus. The positive rate of ABCG2 was 21.2% (7/33), with 30.77% (4/13) in solid type and 15.0% (3/20) in adeno-tubiform type. There was no significant difference between them (P = 0.393). Many positive cells surrounded the vessels in tumor tissues. There were no significant differences between different prognosis groups of these surface phenotypes. The correlative analysis results of three surface phenotypes showed that CD44(+) cells have positive correlation with CD133(+) cells (Spearman, r(s) = 0.416, P = 0.016). In six transplanted tumors of nude mice, the number of positive cases for CD44(+), CD133(+) and ABCG2(+) was 1, 1 and 4 cases, respectively.
The expression of CD44, CD133 and ABCG2 in ACC may influence the progress of ACC. However, they cannot be used as the markers for the evaluation of the prognosis of this tumor.
观察CD44、CD133和ABCG2在眼眶腺样囊性癌（ACC）中的表达及分布情况，并探讨它们与病理类型及预后的相关性。
采用免疫组织化学染色两步法，对33例人眼眶ACC手术石蜡包埋标本、5例复发标本、3例非炎症及肿瘤性疾病切除的泪腺标本以及6例裸鼠移植瘤进行检测。对1991年1月至2009年3月收集的这些患者的临床资料进行回顾性分析。
CD44阳性率为54.5%（18/33），实体型中为76.9%（10/13），腺管状型中为40.0%（8/20）。两者之间差异无统计学意义（P = 0.072）。在实体型中，阳性表达细胞常位于癌巢边缘部分。在腺管状型中，阳性细胞常位于管状结构外层（肌上皮细胞）。CD44在正常组织中也有表达。CD133阳性率为57.6%（19/33），实体型中为76.9%（10/13），腺管状型中为45.0%（9/20）。两者之间差异无统计学意义（P = 0.087）。CD133抗原在细胞质或细胞核中表达，或在细胞质和细胞核中均表达。ABCG2阳性率为21.2%（7/33），实体型中为30.77%（4/13），腺管状型中为15.0%（3/20）。两者之间差异无统计学意义（P = 0.393）。肿瘤组织中许多阳性细胞围绕血管。这些表面表型在不同预后组之间无显著差异。三种表面表型的相关性分析结果显示，CD44(+)细胞与CD133(+)细胞呈正相关（Spearman秩相关系数，r(s) = 0.416，P = 0.016）。在6例裸鼠移植瘤中，CD44(+)、CD133(+)和ABCG2(+)阳性病例数分别为1例、1例和4例。
CD44、CD133和ABCG2在ACC中的表达可能影响ACC的进展。然而，它们不能作为评估该肿瘤预后的标志物。
