National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
J Clin Microbiol. 2012 Mar;50(3):651-6. doi: 10.1128/JCM.05084-11. Epub 2011 Dec 14.
The International Circumpolar Surveillance (ICS) program was initiated in 1999 to conduct population-based surveillance for invasive pneumococcal disease in select regions of the Arctic. The program was expanded to include the surveillance of invasive diseases caused by Neisseria meningitidis and Haemophilus influenzae. An interlaboratory quality control (QC) program to monitor laboratory proficiencies in the serogrouping of N. meningitidis and serotyping of H. influenzae strains was codeveloped by the Arctic Investigations Program (Anchorage, AK) and the Public Health Agency of Canada National Microbiology Laboratory (Winnipeg, Manitoba, Canada) and introduced into the ICS program in 2005. Other participating laboratories included the Provincial Laboratory for Public Health (Edmonton, Alberta, Canada), Laboratoire Santé Publique du Québec (Sainte-Anne-de-Bellevue, Québec, Canada), and Statens Serum Institut (Copenhagen, Denmark). From 2005 through 2009, 50 isolates (24 N. meningitidis and 26 H. influenzae isolates) were distributed among the five participating laboratories. The overall serogroup concordance for N. meningitidis strains was 92.3% (96/104), without including three isolates that were found to express both serogroup Y and W135 specificities. Concordant results were obtained for serogroups A, B, C, and Y among all laboratories. Discrepancies were observed most frequently for serogroups W135, X, Z, and 29E. The overall serotype concordance for H. influenzae was 98% (125/127 attempts). The two discrepant results involved a serotype c strain and a serotype e strain, and in both cases, the serotypeable H. influenzae isolates were misidentified as being nontypeable. These data demonstrate a high degree of concordance for serogroup and serotype determinations of N. meningitidis and H. influenzae isolates, respectively, among the five laboratories participating in this quality control program.
国际极区监测计划（ICS）于 1999 年启动，旨在对北极地区选定地区的侵袭性肺炎球菌病进行基于人群的监测。该计划扩大到包括对脑膜炎奈瑟菌和流感嗜血杆菌引起的侵袭性疾病的监测。一个实验室间质量控制（QC）计划由北极调查计划（安克雷奇，AK）和加拿大公共卫生署国家微生物学实验室（温尼伯，马尼托巴，加拿大）共同制定，以监测脑膜炎奈瑟菌血清群分型和流感嗜血杆菌菌株的血清分型实验室能力，并于 2005 年引入 ICS 计划。其他参与实验室包括省级公共卫生实验室（埃德蒙顿，艾伯塔，加拿大），魁北克公共卫生实验室（Sainte-Anne-de-Bellevue，魁北克，加拿大）和 Statens Serum Institut（哥本哈根，丹麦）。2005 年至 2009 年，50 株分离物（24 株脑膜炎奈瑟菌和 26 株流感嗜血杆菌分离物）分发给五个参与实验室。脑膜炎奈瑟菌菌株的总体血清群一致性为 92.3%（96/104），不包括三个被发现同时表达血清群 Y 和 W135 特异性的分离物。所有实验室均获得 A、B、C 和 Y 血清群的一致结果。血清群 W135、X、Z 和 29E 观察到最频繁的差异。流感嗜血杆菌的总体血清型一致性为 98%（125/127 次尝试）。两个不一致的结果涉及血清型 c 株和血清型 e 株，在这两种情况下，可分型的流感嗜血杆菌分离物被错误识别为不可分型。这些数据表明，参与该质量控制计划的五个实验室中，脑膜炎奈瑟菌和流感嗜血杆菌分离物的血清群和血清型测定具有高度一致性。
