SOX7 参与了阿司匹林介导的人结肠直肠癌细胞生长抑制。
SOX7 is involved in aspirin-mediated growth inhibition of human colorectal cancer cells.
机构信息
The Institute of Genetics and Cytology, School of Life Science, Northeast Normal University, Changchun 130024, Jilin Province, China.
出版信息
World J Gastroenterol. 2011 Nov 28;17(44):4922-7. doi: 10.3748/wjg.v17.i44.4922.
AIM
To confirm the role of sex-determining region Y-box 7 (Sox7) in aspirin-mediated growth inhibition of COX-independent human colorectal cancer cells.
METHODS
The cell survival percentage was examined by MTT (Moto-nuclear cell direc cytotoxicity) assay. SOX7 expression was assessed by using reverse transcription-polymerase chain reaction and Western blotting. SB203580 was used to inhibit the p38MAPK signal pathway. SOX7 promoter activity was detected by Luciferase reporter assay.
RESULTS
SOX7 was upregulated by aspirin and was involved in aspirin-mediated growth inhibition of SW480 human colorectal cancer cells. The p38MAPK pathway played a role in aspirin-induced SOX7 expression, during which the AP1 transcription factors c-Jun and c-Fos upregulated SOX7 promoter activities.
RESULTS
SOX7 is upregulated by aspirin and is involved in aspirin-mediated growth inhibition of human colorectal cancer SW480 cells.
目的
确认性别决定区 Y 框 7（Sox7）在阿司匹林介导的 COX 非依赖性人结肠直肠癌细胞生长抑制中的作用。
方法
通过 MTT（Moto-核细胞直接细胞毒性）检测细胞存活率。通过逆转录-聚合酶链反应和 Western blot 评估 Sox7 表达。使用 SB203580 抑制 p38MAPK 信号通路。通过荧光素酶报告基因检测 Sox7 启动子活性。
结果
阿司匹林上调 Sox7，并参与阿司匹林介导的人结肠直肠癌细胞 SW480 的生长抑制。p38MAPK 通路在阿司匹林诱导的 Sox7 表达中起作用，在此过程中，AP1 转录因子 c-Jun 和 c-Fos 上调 Sox7 启动子活性。
结果
Sox7 被阿司匹林上调，并参与阿司匹林介导的人结肠直肠癌细胞 SW480 的生长抑制。
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