Cooper A G, Codington J F, Miller D K, Brown M C
J Natl Cancer Inst. 1979 Jul;63(1):163-9.
We described the in vivo conversion of the strain-specific ascites murine mammary adenocarcinoma subline TA3-St to a new ascites subline designated TA3-MM. This conversion occurred during passage in a syngeneic A/HeHa mouse infected with pneumonia-producing microorganisms. The mode number of chromosomes of the TA3-MM cell (82) was greater than that of the parental TA3-St cell (69) or the other non-strain-specific subline TA3-Ha (42). The TA3-MM subline could grow in and kill mice of various allogeneic strains. In addition, the TA3-MM cell possessed numerous receptors for the lectin of Vicia graminea seeds, which were hardly detectable at the surface of the parent TA3-St subline but were present in abundance at the cell surface of the non-strain-specific subline TA3-Ha. These lectin receptors of the TA3-Ha cell were previously demonstrated to be present in a unique high-molecular-weight endogenous cell surface glycoprotein termed epiglycanin. The V. gramines lectin receptors on the new TA3-MM subline also were present on an epiglycanin-like molecule. This finding provides further evidence for the hypothesis that allogeneic growth in the TA3 system is a direct result of these membrane glycoproteins.
我们描述了菌株特异性腹水型小鼠乳腺腺癌亚系TA3-St在体内转化为一个新的腹水亚系TA3-MM的过程。这种转化发生在同基因A/HeHa小鼠体内传代过程中，该小鼠感染了能产生肺炎的微生物。TA3-MM细胞的染色体众数（82条）大于亲代TA3-St细胞（69条）或另一个非菌株特异性亚系TA3-Ha（42条）的染色体众数。TA3-MM亚系能够在各种异基因品系的小鼠体内生长并致其死亡。此外，TA3-MM细胞拥有大量野豌豆种子凝集素的受体，这些受体在亲代TA3-St亚系细胞表面几乎检测不到，但在非菌株特异性亚系TA3-Ha的细胞表面大量存在。TA3-Ha细胞的这些凝集素受体先前已被证明存在于一种独特的高分子量内源性细胞表面糖蛋白中，称为表位糖蛋白。新的TA3-MM亚系上的野豌豆凝集素受体也存在于一种类似表位糖蛋白的分子上。这一发现为TA3系统中的异基因生长是这些膜糖蛋白的直接结果这一假说提供了进一步的证据。
