Tanih Nicoline F, Ndip Lucy M, Ndip Roland N
Microbial Pathogenicity and Molecular Epidemiology Research Group, Department of Biochemistry and Microbiology, Faculty of Science and Agriculture, University of Fort Hare, P/Bag X1314, Alice 5700, South Africa; E-Mail:
Int J Mol Sci. 2011;12(11):7459-68. doi: 10.3390/ijms12117459. Epub 2011 Oct 31.
Sequence diversity and population structures can vary widely among pathogenic bacteria species. In some species, all isolates are highly similar, whereas in others most of the isolates are distinguished easily. H. pylori is known for its wide genetic diversity amongst the various strains most especially in the genes involved in virulence. The aim of this study was to evaluate by PCR and sequence analysis, the genetic profile of H. pylori vacA gene (s1, s2, m1 and m2). We sequenced small DNA segments from 13 vacAs1, 10 vacAm2, 6 vacAm1 and 6 vacAs2 strains which were amplified with amplicon size of 259/286 bp, 290 bp and 352 bp for vacAs1/s2, m1 and m2 respectively. Based on similarities among our strains accession numbers were provided for seven vacAs1 (HQ709109-HQ709115), six vacAs2 (JN848463-JN848468), six vacAm1 (JN848469-JN848474) and six vacAm2 (HQ650801-HQ650806) strains. Amongst the strains studied, 98.07%, 98.58%, 97.38% and 95.41% of vacAs1, vacAs2, vacAm1 and vacAm2 of the strains were conserved respectively. Findings of this study underscores the importance of understanding the virulence composition and diversity of H. pylori in South Africa for enhanced clinico-epidemiological monitoring and pathophysiology of disease.
致病细菌物种之间的序列多样性和种群结构可能有很大差异。在某些物种中，所有分离株都高度相似，而在其他物种中，大多数分离株很容易区分。幽门螺杆菌以其在各种菌株中的广泛遗传多样性而闻名，尤其是在与毒力相关的基因中。本研究的目的是通过PCR和序列分析评估幽门螺杆菌vacA基因（s1、s2、m1和m2）的遗传特征。我们对13个vacAs1、10个vacAm2、6个vacAm1和6个vacAs2菌株的小DNA片段进行了测序，这些片段分别用259/286 bp、290 bp和352 bp的扩增子大小进行扩增，用于vacAs1/s2、m1和m2。根据我们菌株之间的相似性，为7个vacAs1（HQ709109-HQ709115）、6个vacAs2（JN848463-JN848468）、6个vacAm1（JN848469-JN848474）和6个vacAm2（HQ650801-HQ650806）菌株提供了登录号。在所研究的菌株中，vacAs1、vacAs2、vacAm1和vacAm2菌株分别有98.07%、98.58%、97.38%和95.41%是保守的。本研究结果强调了了解南非幽门螺杆菌毒力组成和多样性对于加强临床流行病学监测和疾病病理生理学的重要性。
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