[脱氢表雄酮对成骨细胞系的选择性雌激素受体β作用]

[Selective ERβ action of dehydroepiandrosterone for osteoblast cell line].

作者信息

Wang Yu-dong, Lang Xiao, Tao Min-fang, Cheng Wei-wei

机构信息

Department of Gynecology, Shanghai Jiaotong University, Shanghai, China.

出版信息

Zhonghua Fu Chan Ke Za Zhi. 2011 Sep;46(9):674-7.

PMID:22176992

Abstract

OBJECTIVE

To investigate the selective mechanism of dehydroepiandrosterone (DHEA) for osteoblast via ERβ.

METHODS

High expression of ERβ in hMG63-ERβ group (infected with pWPT-ERβ), gene silencing of ERβ in hMG63-shERβ group (infected with pLVTHM-GFP/ERβ-shRNA) and hMG63 group (control) were cultured and treated with 1×10(-7) mol/L DHEA, with or without U0126 and etoposide. The proliferation and apoptosis of hMG63 were evaluated by flow cytometry. The mRNA level of estrogen receptor subtype was detected by reverse transcription-PCR.

RESULTS

The expression of ERβ in hMG63-ERβ group and hMG63-shERβ group were increased 7.39 times and decreased 17% compared with that in hMG63 group (control). DHEA could increase ERβ expression in hMG63 in each group, however, it did not influence the expression of ERα mRNA. When the level of ERβ was high, DHEA could accelerate the proliferation [proliferation index were (81.6 ± 7.6)% in hMG63-ERβ, (75.0 ± 5.3)% in hMG63, P < 0.05] and inhibit the apoptosis [apoptosis rate were (12.2 ± 1.6)% in hMG63-ERβ, (14.6 ± 1.5)% in hMG63, P < 0.01], which was blocked by U0126 [proliferation index were (33.2 ± 2.0)% in hMG63-ERβ, (41.2 ± 2.4)% in hMG63, apoptosis rate were (40.5 ± 4.3)% in hMG63-ERβ, (43.3 ± 4.1)% in hMG63, all P < 0.05]. When the expression of ERβ was silenced, DHEA could not inhibit the apoptosis of hMG63 anymore.

CONCLUSION

DHEA selectively act on osteoblasts via the dominant expression of ERβ.

摘要

目的

探讨脱氢表雄酮（DHEA）通过雌激素受体β（ERβ）对成骨细胞的选择性作用机制。

方法

培养人成骨肉瘤细胞系hMG63，分别构建hMG63-ERβ组（感染pWPT-ERβ）使ERβ高表达、hMG63-shERβ组（感染pLVTHM-GFP/ERβ-shRNA）使ERβ基因沉默及hMG63组（对照组），用1×10⁻⁷mol/L DHEA处理，同时或不同时加入U0126及依托泊苷。采用流式细胞术检测hMG63细胞的增殖和凋亡情况。采用逆转录-聚合酶链反应检测雌激素受体亚型的mRNA水平。

结果

hMG63-ERβ组和hMG63-shERβ组ERβ表达与hMG63组（对照组）相比分别升高7.39倍和降低17%。DHEA可使各组hMG63细胞中ERβ表达增加，但不影响ERα mRNA表达。ERβ高表达时，DHEA可促进hMG63细胞增殖[hMG63-ERβ组增殖指数为(81.6±7.6)%，hMG63组为(75.0±5.3)%，P<0.05]并抑制凋亡[hMG63-ERβ组凋亡率为(12.2±1.6)%，hMG63组为(14.6±1.5)%，P<0.01]，U0126可阻断此作用[hMG63-ERβ组增殖指数为(33.2±2.0)%，hMG63组为(41.2±2.4)%，hMG63-ERβ组凋亡率为(40.5±4.3)%，hMG63组为(43.3±4.1)%，均P<0.05]。ERβ基因沉默时，DHEA不再能抑制hMG63细胞凋亡。