使用放射性同位素 PAHA 盘测量零食的致龋性。
Measurement of the cariogenicity of snacks using a radioisotope PAHA disc.
机构信息
Department of Preventive & Social Dentistry, School of Dentistry, Seoul National University, South Korea.
出版信息
Arch Oral Biol. 2012 Jun;57(6):769-74. doi: 10.1016/j.archoralbio.2011.11.020. Epub 2011 Dec 15.
OBJECTIVES
This study aimed to establish a method for measuring the cariogenic potential of foods with high reproducibility in vitro.
METHODS
Streptococcus mutans (S. mutans) was incubated in test foods with radioisotope polyacrylamide hydroxyapatite (PAHA) for 150 min at 37 °C. Then, the amount of radioisotope (32)P released from PAHA was measured using a liquid scintillation counter and scanning electron microscopy (SEM).
RESULTS
The radioisotope PAHA discs that were soaked in 10% sucrose solutions had a high cariogenic potential and showed a remarkably demineralized surface (p < 0.05). The radioisotope PAHA disc that was incubated with snacks that had a high cariogenic potential showed a remarkably demineralized surface via SEM. Candy had a relatively high cariogenic potential, whereas xylitol gum had a relatively low potential.
CONCLUSIONS
The cariogenicity of snacks can easily be evaluated by measuring the amount of (32)P released from radioisotope PAHA discs.
目的
本研究旨在建立一种具有高重复性的体外测量食物致龋潜力的方法。
方法
将变异链球菌（S. mutans）在含有放射性同位素聚丙稀酰胺羟磷灰石（PAHA）的试验食品中于 37°C 下孵育 150 分钟。然后，使用液体闪烁计数器和扫描电子显微镜（SEM）测量从 PAHA 释放的放射性同位素（32）P 的量。
结果
浸泡在 10%蔗糖溶液中的放射性同位素 PAHA 圆盘具有高致龋潜力，表面明显脱矿（p<0.05）。通过 SEM 观察到，与具有高致龋潜力的零食一起孵育的放射性同位素 PAHA 圆盘表面明显脱矿。糖果具有相对较高的致龋潜力，而木糖醇口香糖的致龋潜力相对较低。
结论
通过测量从放射性同位素 PAHA 圆盘释放的（32）P 的量，很容易评估零食的致龋性。
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