光生物反应器策略提高土著斜生栅藻 CNW-N 的 CO2 固定效率：CO2 进料、光照和操作方式的统计优化。
Photobioreactor strategies for improving the CO2 fixation efficiency of indigenous Scenedesmus obliquus CNW-N: statistical optimization of CO2 feeding, illumination, and operation mode.
机构信息
Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan.
出版信息
Bioresour Technol. 2012 Feb;105:106-13. doi: 10.1016/j.biortech.2011.11.091. Epub 2011 Dec 1.
Statistical experimental design and bioreactor strategies were applied to enhance CO(2) fixation ability of microalga Scenedesmus obliquus CNW-N. Four operating parameters strongly influencing microalgal CO(2) fixation efficiency (namely, CO(2) concentration, CO(2) flow rate, magnesium concentration, and light intensity) were optimized with response surface methodology. The optimal range of parameters achieving the best overall performance of specific growth rate and CO(2) fixation rate was determined with overlay counter plot techniques. Optimal ranges of CO(2) concentration, CO(2) flow rate, magnesium concentration and light intensity were 2.0-2.5%, 0.3-0.5 vvm, 1.7-2.7 mM and 180-250 μmol m(-2) s(-1), respectively, achieving a specific growth rate of >1.22 d(-1) and CO(2) fixation rate of >800 mg L(-1) d(-1). Semi-batch operations further enhanced the biomass productivity, photosynthesis efficiency, and CO(2) fixation rate to 1030 mg L(-1) d(-1), 10.5%, and 1782 mg L(-1) d(-1), respectively. This performance is better than the results reported by most related studies.
采用统计实验设计和生物反应器策略来提高斜生栅藻 CNW-N 的 CO2 固定能力。利用响应面法对强烈影响微藻 CO2 固定效率的四个操作参数（即 CO2 浓度、CO2 流速、镁浓度和光照强度）进行了优化。利用叠加对比绘图技术确定了达到最佳比生长速率和 CO2 固定速率总体性能的最佳参数范围。CO2 浓度、CO2 流速、镁浓度和光照强度的最佳范围分别为 2.0-2.5%、0.3-0.5 vvm、1.7-2.7 mM 和 180-250 μmol m(-2) s(-1)，实现了 >1.22 d(-1)的比生长速率和 >800 mg L(-1) d(-1)的 CO2 固定速率。半分批操作进一步提高了生物量生产力、光合作用效率和 CO2 固定率，分别达到 1030 mg L(-1) d(-1)、10.5%和 1782 mg L(-1) d(-1)。这一性能优于大多数相关研究的结果。
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