Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan.
Bioresour Technol. 2012 Jun;113:244-52. doi: 10.1016/j.biortech.2011.11.133. Epub 2011 Dec 8.
Engineering strategies were applied to improve the CO(2) fixation rate and carbohydrate/lipid production of a Scenedesmus obliquus CNW-N isolate. The light intensity that promotes cell growth, carbohydrate/lipid productivity, and CO(2) fixation efficiency was identified. Nitrogen starvation was also employed to trigger the accumulation of lipid and carbohydrate. The highest productivity of biomass, lipid, and carbohydrate was 840.57 mg L(-1)d(-1), 140.35 mg L(-1)d(-1). The highest lipid and carbohydrate content was 22.4% (5-day N-starvation) and 46.65% (1-day N-starvation), respectively. The optimal CO(2) consumption rate was 1420.6 mg L(-1)d(-1). This performance is better than that reported in most other studies. Under nitrogen starvation, the microalgal lipid was mainly composed of C16/C18 fatty acid (around 90%), which is suitable for biodiesel synthesis. The carbohydrate present in the biomass was mainly glucose, accounting for 77-80% of total carbohydrates. This carbohydrate composition is also suitable for fermentative biofuels production (e.g., bioethanol and biobutanol).
采用工程策略来提高斜生栅藻 CNW-N 分离株的 CO2 固定率和碳水化合物/脂质产量。确定了促进细胞生长、碳水化合物/脂质生产率和 CO2 固定效率的最佳光照强度。还采用氮饥饿来触发脂质和碳水化合物的积累。生物质、脂质和碳水化合物的最高生产力分别为 840.57mg/L/d、140.35mg/L/d。最高的脂质和碳水化合物含量分别为 22.4%（5 天氮饥饿）和 46.65%（1 天氮饥饿）。最佳的 CO2 消耗速率为 1420.6mg/L/d。该性能优于大多数其他研究报告的结果。在氮饥饿条件下，微藻脂质主要由 C16/C18 脂肪酸组成（约 90%），这适合生物柴油的合成。生物量中存在的碳水化合物主要是葡萄糖，占总碳水化合物的 77-80%。这种碳水化合物组成也适合发酵生物燃料的生产（例如，生物乙醇和生物丁醇）。
