Guangzhou Women and Children's Medical Center, Guangzhou Medical College, Guangzhou, People's Republic of China.
Can J Microbiol. 2012 Jan;58(1):112-5. doi: 10.1139/w11-112. Epub 2011 Dec 19.
New Delhi metallo-β-lactamase-1 (NDM-1) is a novel type of metallo-β-lactamase (MBL) responsible for bacterial resistance to β-lactam antibiotics. Acinetobacter junii was previously shown to possess a MBL phenotype; however, the genes responsible for this phenotype were not identified. In this study, we reported the identification of NDM-1 gene in a clinical isolate of A. junii from a child patient in China, which was resistant to all β-lactams except aztreonam but sensitive to aminoglycosides and quinolones. The cloned NDM-1 gene contained an open reading frame of 813 bp and had a nucleotide sequence 99.9% identical (812/813) to reported NDM-1 genes carried by Acinetobacter baumannii , Enterococcus faecium , Escherichia coli , and Klebsiella pneumoniae . Recombinant NDM-1 protein was successfully expressed in E. coli BL21, and antibiotic sensitivities of the NDM-1-producing E. coli were largely similar to the A. junii 1454 isolate. The findings of this study raise attention to the emergence and spread of NDM-1-carrying bacteria in China.
新德里金属β-内酰胺酶-1（NDM-1）是一种新型的金属β-内酰胺酶（MBL），可导致细菌对β-内酰胺类抗生素产生耐药性。先前已经证明琼氏不动杆菌具有 MBL 表型；然而，尚未鉴定出导致这种表型的基因。在这项研究中，我们报道了在中国的一名儿童患者的临床分离株琼氏不动杆菌中发现 NDM-1 基因，该菌对除氨曲南以外的所有β-内酰胺类抗生素均耐药，但对氨基糖苷类和喹诺酮类敏感。克隆的 NDM-1 基因包含 813bp 的开放阅读框，其核苷酸序列与报道的鲍曼不动杆菌、屎肠球菌、大肠杆菌和肺炎克雷伯菌携带的 NDM-1 基因 99.9%（812/813）相同。重组 NDM-1 蛋白在大肠杆菌 BL21 中成功表达，产 NDM-1 的大肠杆菌的抗生素敏感性与琼氏不动杆菌 1454 分离株大致相似。本研究结果引起了对中国携带 NDM-1 细菌的出现和传播的关注。
