Department of Analytical Chemistry, Institute of Chemical Technology in Prague, 166 28 Prague, The Czech Republic.
Biomacromolecules. 2012 Feb 13;13(2):489-98. doi: 10.1021/bm2015366. Epub 2011 Dec 29.
Polysaccharide films containing chitosan, methylcellulose, and a mixture of these polysaccharides in various ratios were prepared and modified with meso-tetrakis(4-sulfonatophenyl)porphyrin in an aqueous medium at pH 7. The modified films were compared with the initial films using spectroscopic methods and microscopic imaging. Electronic (UV-vis absorption, electronic circular dichroism (ECD)) and vibrational (FTIR and Raman) spectra showed that the porphyrin macrocycles had a strong affinity toward chitosan and did not interact with the methylcellulose. The total porphyrin uptake depended on the chitosan: methylcellulose ratio and pure methylcellulose films did not retain porphyrin macrocycles. ECD measurements detected the presence of optically active porphyrin species bound to the films. SEM and AFM images confirmed that the porphyrin macrocycles caused structural changes on the film surface and within the film layer.
制备了含有壳聚糖、甲基纤维素以及这些多糖以不同比例混合的多糖薄膜，并在 pH 值为 7 的水介质中用间四(4-磺基苯基)卟啉进行修饰。使用光谱方法和微观成像对修饰后的薄膜与初始薄膜进行了比较。电子（紫外-可见吸收、电子圆二色性（ECD））和振动（FTIR 和拉曼）光谱表明卟啉大环对壳聚糖具有很强的亲和力，而与甲基纤维素不相互作用。卟啉大环的总摄取量取决于壳聚糖与甲基纤维素的比例，而纯甲基纤维素薄膜不保留卟啉大环。ECD 测量检测到结合在薄膜上的具有光活性的卟啉物种的存在。SEM 和 AFM 图像证实，卟啉大环在薄膜表面和薄膜层内引起了结构变化。
