通过有针对性地选择试剂和操作参数来优化电子转移解离。
Optimization of electron transfer dissociation via informed selection of reagents and operating parameters.
机构信息
Department of Chemistry, University of Virginia, McCormick Rd., Charlottesville, Virginia 22904, USA.
出版信息
Anal Chem. 2012 Feb 7;84(3):1781-5. doi: 10.1021/ac202807h. Epub 2012 Jan 6.
Abstract
Electron transfer dissociation (ETD) has improved the mass spectrometric analysis of proteins and peptides with labile post-translational modifications and larger intact masses. Here, the parameters governing the reaction rate of ETD are examined experimentally. Currently, due to reagent injection and isolation events as well as longer reaction times, ETD spectra require significantly more time to acquire than collision-induced dissociation (CID) spectra (>100 ms), resulting in a trade-off in the dynamic range of tandem MS analyses when ETD-based methods are compared to CID-based methods. Through fine adjustment of reaction parameters and the selection of reagents with optimal characteristics, we demonstrate a drastic reduction in the time taken per ETD event. In fact, ETD can be performed with optimal efficiency in nearly the same time as CID at low precursor charge state (z = +3) and becomes faster at higher charge state (z > +3).
摘要
电子转移解离（ETD）提高了对具有不稳定翻译后修饰和较大完整质量的蛋白质和肽的质谱分析。在这里，实验研究了控制 ETD 反应速率的参数。目前，由于试剂注入和分离事件以及较长的反应时间，ETD 谱比碰撞诱导解离（CID）谱需要更长的时间来获取（>100ms），这导致在串联质谱分析中，当与 CID 方法相比时，基于 ETD 的方法的动态范围存在权衡。通过精细调整反应参数和选择具有最佳特性的试剂，我们证明每个 ETD 事件所需的时间大大减少。实际上，在低前体电荷状态（z=+3）下，ETD 可以以接近 CID 的效率几乎在相同的时间内进行，并且在较高的电荷状态（z>+3）下变得更快。
相似文献
1
Optimization of electron transfer dissociation via informed selection of reagents and operating parameters.
Anal Chem. 2012 Feb 7;84(3):1781-5. doi: 10.1021/ac202807h. Epub 2012 Jan 6.
2
On-line LC-MS approach combining collision-induced dissociation (CID), electron-transfer dissociation (ETD), and CID of an isolated charge-reduced species for the trace-level characterization of proteins with post-translational modifications.
J Proteome Res. 2007 Nov;6(11):4230-44. doi: 10.1021/pr070313u. Epub 2007 Sep 28.
3
Improved peptide identification for proteomic analysis based on comprehensive characterization of electron transfer dissociation spectra.
J Proteome Res. 2010 Dec 3;9(12):6354-67. doi: 10.1021/pr100648r. Epub 2010 Nov 12.
4
On performing simultaneous electron transfer dissociation and collision-induced dissociation on multiply protonated peptides in a linear ion trap.
J Am Soc Mass Spectrom. 2009 Sep;20(9):1672-83. doi: 10.1016/j.jasms.2009.05.009. Epub 2009 May 20.
5
Comprehensive comparison of collision induced dissociation and electron transfer dissociation.
Anal Chem. 2008 Jul 1;80(13):4825-35. doi: 10.1021/ac8007785. Epub 2008 Jun 10.
6
High-energy electron transfer dissociation (HE-ETD) using alkali metal targets for sequence analysis of post-translational peptides.
J Am Soc Mass Spectrom. 2010 Sep;21(9):1482-9. doi: 10.1016/j.jasms.2010.05.010. Epub 2010 Jun 9.
7
Electron transfer dissociation of iTRAQ labeled peptide ions.
J Proteome Res. 2008 Sep;7(9):3643-8. doi: 10.1021/pr8001113. Epub 2008 Jul 23.
8
Effects of electron-transfer coupled with collision-induced dissociation (ET/CID) on doubly charged peptides and phosphopeptides.
J Am Soc Mass Spectrom. 2011 Jan;22(1):57-66. doi: 10.1007/s13361-010-0020-9. Epub 2011 Jan 27.
9
Large-scale identification of endogenous secretory peptides using electron transfer dissociation mass spectrometry.
Mol Cell Proteomics. 2013 Mar;12(3):700-9. doi: 10.1074/mcp.M112.017400. Epub 2012 Dec 18.
10
Multiplexed Comparative Analysis of Intact Glycopeptides Using Electron-Transfer Dissociation and Synchronous Precursor Selection Based Triple-Stage Mass Spectrometry.
Anal Chem. 2020 Jun 2;92(11):7547-7555. doi: 10.1021/acs.analchem.0c00014. Epub 2020 May 14.
引用本文的文献
1
Evaluating iron diimines: ion-pairing, lability and the reduced state.
Phys Chem Chem Phys. 2025 Apr 9;27(15):7882-7892. doi: 10.1039/d5cp00199d.
2
The Hunt Lab Weighs in on Mass Spectrometry-Based Analysis of Protein Posttranslational Modifications.
Mol Cell Proteomics. 2025 Apr;24(4):100943. doi: 10.1016/j.mcpro.2025.100943. Epub 2025 Mar 11.
3
The protamines of the spider Steatoda sp. provide an example of liquid-liquid phase separation chromatin transitions during spermiogenesis.
Development. 2024 Nov 15;151(22). doi: 10.1242/dev.203134. Epub 2024 Nov 18.
4
The protamines of the noble false widow spider provide an example of liquid-liquid phase separation chromatin transitions during spermiogenesis.
bioRxiv. 2024 Jun 5:2024.06.04.597381. doi: 10.1101/2024.06.04.597381.
5
Instrumentation at the Leading Edge of Proteomics.
Anal Chem. 2024 May 21;96(20):7976-8010. doi: 10.1021/acs.analchem.3c04497. Epub 2024 May 13.
6
Characterization of Phosphorylated Peptides by Electron-Activated and Ultraviolet Dissociation Mass Spectrometry: A Comparative Study with Collision-Induced Dissociation.
J Am Soc Mass Spectrom. 2024 May 1;35(5):1040-1054. doi: 10.1021/jasms.4c00048. Epub 2024 Apr 16.
7
Localization and Quantification of Post-Translational Modifications of Proteins Using Electron Activated Dissociation Fragmentation on a Fast-Acquisition Time-of-Flight Mass Spectrometer.
J Am Soc Mass Spectrom. 2023 Oct 4;34(10):2199-2210. doi: 10.1021/jasms.3c00144. Epub 2023 Sep 11.
8
Improved Sequence Analysis of Intact Proteins by Parallel Ion Parking during Electron Transfer Dissociation.
Anal Chem. 2021 Nov 30;93(47):15728-15735. doi: 10.1021/acs.analchem.1c03652. Epub 2021 Nov 17.
9
Nitrogen-Containing Aromatic Radical Anions Perform Multiple Proton and Electron Transfers Near-Simultaneously with Multiply Protonated Cations.
Anal Chem. 2021 Nov 2;93(43):14365-14368. doi: 10.1021/acs.analchem.1c03801. Epub 2021 Oct 20.
10
Top-Down Characterization of an Intact Monoclonal Antibody Using Activated Ion Electron Transfer Dissociation.
Anal Chem. 2020 Aug 4;92(15):10246-10251. doi: 10.1021/acs.analchem.0c00705. Epub 2020 Jul 16.
本文引用的文献
1
Deep and highly sensitive proteome coverage by LC-MS/MS without prefractionation.
Mol Cell Proteomics. 2011 Aug;10(8):M110.003699. doi: 10.1074/mcp.M110.003699. Epub 2011 May 17.
2
Fourier transform ion cyclotron resonance cell with dynamic harmonization of the electric field in the whole volume by shaping of the excitation and detection electrode assembly.
Rapid Commun Mass Spectrom. 2011 Jan 15;25(1):122-6. doi: 10.1002/rcm.4838.
3
A dual pressure linear ion trap Orbitrap instrument with very high sequencing speed.
Mol Cell Proteomics. 2009 Dec;8(12):2759-69. doi: 10.1074/mcp.M900375-MCP200. Epub 2009 Oct 14.
4
Performance evaluation of a high-field Orbitrap mass analyzer.
J Am Soc Mass Spectrom. 2009 Aug;20(8):1391-6. doi: 10.1016/j.jasms.2009.01.005. Epub 2009 Jan 18.
5
Methods for analyzing peptides and proteins on a chromatographic timescale by electron-transfer dissociation mass spectrometry.
Nat Protoc. 2008;3(11):1709-17. doi: 10.1038/nprot.2008.159.
6
The utility of ETD mass spectrometry in proteomic analysis.
Biochim Biophys Acta. 2006 Dec;1764(12):1811-22. doi: 10.1016/j.bbapap.2006.10.003. Epub 2006 Oct 30.
7
Performance evaluation of a hybrid linear ion trap/orbitrap mass spectrometer.
Anal Chem. 2006 Apr 1;78(7):2113-20. doi: 10.1021/ac0518811.
8
Electron transfer versus proton transfer in gas-phase ion/ion reactions of polyprotonated peptides.
J Am Chem Soc. 2005 Sep 14;127(36):12627-39. doi: 10.1021/ja0526057.
9
Protein identification using sequential ion/ion reactions and tandem mass spectrometry.
Proc Natl Acad Sci U S A. 2005 Jul 5;102(27):9463-8. doi: 10.1073/pnas.0503189102. Epub 2005 Jun 27.
10
Mutual storage mode ion/ion reactions in a hybrid linear ion trap.
J Am Soc Mass Spectrom. 2005 Jan;16(1):71-81. doi: 10.1016/j.jasms.2004.09.017.
Zotero 插件